文章标题：Paris saponin VII suppresses osteosarcoma progression by targeting VEGFR2 and PD-L1 to enhance anti-angiogenic and immunotherapeutic efficacy

作者列表：Ma Zuyi, Sun Jia, Wu Xin, Shang Changzhen, Li Binglu

影响因子：6

期刊：Cancer Cell International

发表时间：2026-4-6

DOI:10.1186/s12935-026-04289-0

文献主题：Abstract

Objective

To investigate the inhibitory effects and underlying mechanisms of Paris saponin VII (PS VII) on osteosarcoma (OS) progression.

Methods

Human osteosarcoma MG63 cells were treated with PS VII at concentrations of 0, 1, and 10 µmol·L⁻¹. Cell viability was assessed using the Cell Counting Kit-8 (CCK-8) assay, and clonogenic capacity was evaluated by colony ｆormation assays. Potential molecular targets of PS VII were predicted using the SwissTargetPrediction database. Intersection targets between PS VII-related targets and OS differentially expressed genes (DEGs) were identified, followed by protein–protein interaction (PPI) network construction to screen core targets. Molecular docking was perｆormed to validate compound–target interactions. Western blotting was used to verify the regulatory effects of PS VII on key protein targets. In vivo, a K7M2 murine osteosarcoma subcutaneous xenograft model was established to evaluate tumor growth and therapeutic synergy with bevacizumab or anti-PD-1 antibody.