产品编号Rsm-10801M 英文名称Adiponectin 中文名称脂联素单克隆抗体 别 名30 kDa adipocyte complement related protein; 30 kDa adipocyte complement-related protein; ACDC; ACRP 30; ACRP30; ADIPO_HUMAN; Adipocyte; Adipocyte C1q ａnd collagen domain containing protein; Adipocyte complement related 30 kDa protein; Adipocyte complement related protein of 30 kDa; Adipocyte complement-related 30 kDa protein; Adiponectin; AdipoQ; Adipose most abundant gene transcript 1; Adipose most abundant gene transcript 1 protein; Adipose specific collagen like factor; ADIPQTL1; ADPN; APM 1; apM-1; ApM1; C1q ａnd collagen domain-containing protein; GBP 28; GBP28; Gelatin binding protein; Gelatin binding protein 28; Gelatin-binding protein. 说 明 书0.1ml 0.2ml 研究领域免疫学 糖尿病 内分泌病 抗体来源Mouse 克隆类型Monoclonal 克 隆 号3B5 交叉反应Human, 产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分 子 量25kDa 细胞定位分泌型蛋白 性 状Lyophilized or Liquid 浓 度1mg/1ml 免 疫 原Recombinant human Adiponectin full length protein 亚 型IgG 纯化方法affinity purified by Protein A 储 存 液0.01M TBS（pH7.4） with 1% BSA, 0.03% Proclin300 ａnd 50% Glycerol. 保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. PubMedPubMed 产品介绍background: Adiponectin: A protein hormone produced ａnd secreted exclusively by adipocytes （fat cells） that regulates the metabolism of lipids ａnd glucose. Adiponectin influences the body＇s response to insulin. Adiponectin also has antiinflammatory effects on the cells lining the walls of blood vessels. High blood levels of adiponectin are associated with a reduced risk of heart attack. Low levels of adiponectin are found in people who are obese （and who are at increased risk of a heart attack）. Adipocytes produce ａnd secrete a number of proteins, including leptin, adipsin, properdin, ａnd tumor necrosis factor （TNF）. An adipose tissue-specific factor was isolated ａnd termed APM1 （AdiPose Most abundant gene transcript 1）. The protein product of APM1 is adiponectin. The APM1 gene maps to chromosome 3q27.
Function: Important adipokine involved in the control of fat metabolism ａnd insulin sensitivity, with direct anti-diabetic, anti-atherogenic ａnd anti-inflammatory activities. Stimulates AMPK phosphorylation ａnd activation in the liver ａnd the skeletal muscle, enhancing glucose utilization ａnd fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver ａnd macrophages, ａnd also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis ａnd tissue remodeling by binding ａnd sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
Subunit: Homomultimer. Forms trimers, hexamers ａnd 12- to 18-mers. The trimers （low molecular weight complexes / LMW） are assembled via non-covalent interactions of the collagen-like domains in a triple helix ａnd hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers （middle molecular weight complexes / MMW） ａnd larger complexes （higher molecular weight / HMW）. The HMW-complex assembly may rely aditionally on lysine hydroxylation ａnd glycosylation. LMW, MMW ａnd HMW complexes bind to HBEGF, MMW ａnd HMW complexes bind to PDGFB, ａnd HMW complex binds to FGF2. Interacts with CTRP9A via the C1q domain （heterotrimeric complex）.
Subcellular Location: Secreted.
Tissue Specificity: Synthesized exclusively by adipocytes ａnd secreted into plasma.
Post-translational modifications: Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation ａnd glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes ａnd consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes （By similarity）. O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
DISEASE: Adiponectin deficiency （ADPND） [MIM:612556]: A condition that results in very low concentrations of plasma adiponectin. Note=The disease is caused by mutations affecting the gene represented in this entry. Diabetes mellitus, non-insulin-dependent （NIDDM） [MIM:125853]: A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body＇s own insulin. Affected individuals usually have an obese body habitus ａnd manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension ａnd hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, ａnd blood vessels. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
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