Catalog Number : RSF-105345R-AF488

Quantity size :100ug (2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10mg/ml BSA and 0.1% Sodium azide. Reconstitute with 100ul sterile distilled water. )

background:
eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5＇-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot)

Specificity : · anti-phospho-EIF4B (Ser422) is a Mouse monoclonal antibody, AF488 conjugated

· specific for anti-phospho-EIF4B (Ser422)/AF488 of Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig,

· Protein G affinity chromatography purification, purity :>95%

· Isotype: IgG

· mol wt：69kDa

Application :

·FCM: 1:50-200

·IF : 1:50-1:200

· Immunocytochemistry: 1:50-1:200

· Excitation spectrum: 495nm

· Emission spectrum: 519nm

· Optimal working dilutions must be determined by the end user.

· protect from light.

Storage:Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.