![NR8383细胞[大鼠肺泡巨噬细胞]/菌种](https://img5.app17.com/products/big/20170505/201705050851437440.webp?w=300&h=300)
产品展示
![NR8383细胞[大鼠肺泡巨噬细胞]/菌种](https://img5.app17.com/products/big/20170505/201705050851437440.webp)
NR8383细胞[大鼠肺泡巨噬细胞]/菌种
点击次数：387
价格：￥电议
更新日期：2026/9/8 3:56:46
生 产 地：美洲
产品型号：
- 电话: 13******486
- 手机: 13******486
- 微信:
- 交换名片后展示联系方式 → →
详细内容
细胞运输：干冰运输（1 Vial）或活细胞运输（T-25 flasks）
细胞用途：只可用于科研，不可用于临床诊断和治疗
NR8383细胞,大鼠肺泡巨噬细胞 操作方法
| 固定 | 培养细胞的制片或冰冻切片用4%多聚甲醛固定30min(4℃)后，用80%酒精再固定2h（-20℃）。常规4%中性福尔马林固定、石蜡包埋之切片进行脱蜡、水化。 |
| 洗涤 | 玻片浸入PBS缓冲液，摇床上洗涤5min，三次。 |
| 反应 | 洗涤后的玻片用吸水纸吸干细胞或组织周围水分，按50μl/cm2滴加反应液（每50μL反应液含TdT酶0.5μL,标记的-dUTP 1μL），使反应液均匀地覆盖于所有细胞或组织切片上，盖上塑料盖玻片，置湿盒中，37℃孵育1h。 |
| 终止反应 | 去掉塑料盖玻片，将玻片置盛有洗涤缓冲液的染色缸内，洗涤两次，每次5min。 |
| FITC标记 | 洗涤后的玻片用吸水纸吸去细胞或组织周围水分，按50μl/cm2滴加FITC反应液（含FITC 2.5μg/mL），室温下避光孵育10min。 |
| 洗涤 | 将玻片置于洗涤缓冲液内，洗两次，每次5min。 |
| PI复染 | 将玻片置于盛有PI染液的染色缸内，室温下避光染色30min。 |
| 封片 | 用盖玻片直接盖在含PI染液的玻片上，亦可用无色指甲油涂于盖玻片四周边缘，置暗盒中，尽早镜检观察。 |
| 交货时间 | 10~15天 |
NR8383细胞,大鼠肺泡巨噬细胞 客户须知（对材料的要求、注意事项）
结果提供：正常和阳性对照玻片及其1张数码照片，剩余石蜡块，检测报告；
样本要求：取材保持组织新鲜（组织块以小于2cm×1.5cm×0.3cm为宜），立即放入固定液中（固定液用10%福尔马林液或4%多聚甲醛缓冲液，体积为组织块的10倍以上），避免干燥；对于有血迹的样本，可用PBS液或生理盐水冲洗后直接放入固定液中；经固定后的样本必须在48小时内处理。
一、细胞信息
| Organism | Rattus norvegicus, rat |
|---|---|
| Tissue | lung |
| Cell Type | macrophage (alveolar) |
| Product Format | frozen |
| Morphology | macrophage |
| Culture Properties | mixed, adherent ａnd suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | normal |
| Strain | Sprague-Dawley |
| Applications | The NR8383 cell line provides a homogenous source of highly responsive alveolar macarophages which can be used in vitro to study macrophage related activities. |
| Storage Conditions | liquid nitrogen vapor phase |
二、细胞特点
| Images |  |
|---|---|
| Derivation | NR8383 (normal rat, August 3, 1983) was established from normal rat alveolar macrophage cells obtained by lung lavage. NR8383 cells were cloned ａnd subcloned from single cells by limiting dilution, ａnd then subcloned from soft agar three times. |
| Receptor Expression | Fc |
| Genes Expressed | transforming growth factor beta (TGF beta); interleukin 1 (IL-1); interleukin 6 (IL-6) |
| Cellular Products | transforming growth factor beta (TGF beta); interleukin 1 (IL-1); interleukin 6 (IL-6) |
| Comments | The cells were cultured in the presence of gerbil lung cell conditioned medium for approximately 8 to 9 months. Subsequently the requirement for exogenous growth factors was lost. The cells exhibit characteristics of macrophage cells. Phagocytosis of zymosan ａnd Pseudomonas aeruginosa, nonspecific esterase activity, Fc receptors, oxidative burst,IL-1, TNF beta ａnd IL-6 secretion, ａnd replicative response to exogenous growth factors. The cells respond to appropriate microbial, particulate or soluble stimuli with phagocytosis ａnd killing. NR8383 cells respond to bleomycin by secreting latent transforming growth factor (TGF beta). Stimulation with bleomycin also increases TGF beta mRNA expression. These cells are sensitive to endotoxin. LPS levels of 1 to 10 ng/mL inhibit replication by 50%. LPS inhibition is nontoxic ａnd reversible even after levels up to 0.001 mg/mL for extended periods. |
三、培养方法
| Complete Growth Medium | Ham＇s F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; heat inactivated fetal bovine serum, 15% |
|---|---|
| Subculturing | Cultures can be maintained by transferring floating cells to additional flasks. Adherent cells may be harvested by scraping. Upon reseeding, about one half of the cells will re-attach. Cultures are most successful when set up at a floating cell concentration of 1- 4 x 105 viable cells/mL. Medium Renewal: Two to three times weekly |
| Cryopreservation | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C |
四、发展历史
| Name of Depositor | RJ Helmke |
|---|---|
| Year of Origin | August 3, 1983 |
| References | Hidalgo HA, et al. Pneumocystis carinii induces an oxidative burst in alveolar macrophages. Infect. Immun. 60: 1⁻⁷, 1992. PubMed:1729174 Helmke RJ, et al. A continuous alveolar macrophage cell line: comparisons with freshly derived alveolar macrophages. In Vitro Cell. Dev. Biol. 25: 44-48, 1989. PubMed: 2914814 Helmke RJ, et al. From growth factor dependence to growth factor responsiveness: the genesis of an alveolar macrophage cell line. In Vitro Cell. Dev. Biol. 23: 567-574, 1987. PubMed: 3497918 Limper AH, Standing JE. Vitronectin interacts with Candida albicans ａnd augments organism attachment to the NR8383 macrophage cell line. Immunol. Lett. 42: 139-144, 1994. PubMed:7534269 Hidalgo HA, et al. The effects of cyclosporine ａnd dexamethasone on an alveolar macrophage cell line (NR8383). Transplantation 53: 620-623, 1992. PubMed: 1549855 Denholm EM, Rollins SM. Expression ａnd secretion of transforming growth factor-beta by bleomycin-stimulated rat alveolar macrophages. Am. J. Physiol. 264: L36-L42, 1993. PubMed: 7679254 Krieg DP, et al. Resistance of mucoid Pseudomonas aeruginosa to nonopsonic phagocytosis by alveolar macrophages in vitro. Infect. Immun. 56: 3173-3169, 1988. PubMed: 3141284 Sherman MP, et al. Pyrrolidine dithiocarbamate inhibits induction of nitric oxide synthase activity in rat alveolar macrophages. Biochem. Biophys. Res. Commun. 191: 1301-1308, 1993. PubMed: 7682068 Griscavage JM, et al. Inducible nitric oxide synthase from a rat alveolar macrophage cell line is inhibited by nitric oxide. J. Immunol. 151: 6329-6337, 1993. PubMed: 7504017 Henderson SA, et al. Nitric oxide reduces early growth response-1 gene expression in rat lung macrophages treated with interferon-gamma ａnd lipopolysaccharide. J. Biol. Chem. 269: 25239-25242, 1994. PubMed: 7523382 Huang S, et al. Rat KC cDNA cloning ａnd mRNA expression in lung macrophages ａnd fibroblasts. Biochem. Biophys. Res. Commun. 184: 922-929, 1992. PubMed: 1374243 |
