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小鼠脑神经瘤细胞2A代理
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价格：￥电议
更新日期：2026/9/8 3:56:46
生 产 地：美洲
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相关标签：小鼠脑神经瘤细胞株2A,2A细胞株,小鼠细胞株型号齐全
详细内容
一、产品概述
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| Organism | Mus musculus, mouse |
|---|---|
| Tissue | brain |
| Cell Type | neuroblast |
| Product Format | frozen |
| Morphology | neuronal ａnd amoeboid stem cells |
| Culture Properties | adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | neuroblastoma |
| Strain | A |
| Applications | This cell line is a suitable transfection host. The cell line has been used for studies on the mechanism of vinblastine precipitation of microtubular protein, the kinetics of GTP binding to isolated protein, the turnover of microtubules in vivo, ａnd the synthesis ａnd assembly of microtubular protein . The World Organization for Animal Health (OIE) uses the cells for routine diagnosis of rabies. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | modal number = 95; range = 59 to 193. Karyotype unstable within a stemline range of 94 to 98 chromosomes. All the cells contain 6 to 10 large chromosomes with median or submedian centromeres ａnd 2 to 4 minute chromosomes. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
|---|---|
| Images |  |
| Derivation | Clone Neuro-2a was established by R.J. Klebe ａnd F.H. Ruddle from a spontaneous tumor of a strain A albino mouse. This tumor line, designated C1300, was obtained from the Jackson Laboratory, Bar Harbor, Maine |
| Antigen Expression | H-2, a haplotype; Mus musculus, expressed |
| Genes Expressed | acetylcholinesterase, tubulin |
| Cellular Products | acetylcholinesterase tubulin |
| Virus Susceptibility | Herpes simplex virus Vesicular stomatitis virus Human poliovirus 1 |
| Comments | Neuro-2a cells produce large quantities of microtubular protein which is believed to play a role in a contractile system which is responsible for axoplasmic flow in nerve cells. Tested ａnd found negative for ectromelia virus (mousepox). |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle＇s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T⁻⁷5 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 1 to 2 times per week |
| Cryopreservation | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
小鼠脑神经瘤细胞株2A,2A细胞株,小鼠细胞株型号齐全
