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RAG 小鼠肾腺癌细胞
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价格：￥电议
更新日期：2026/9/8 3:56:46
生 产 地：美洲
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详细内容
资源名称 RAG
种属 小鼠肾腺癌细胞
类型 肾腺癌；肾
RAG小鼠肾腺癌细胞形态 变形虫样
培养方法
培养基 MEM培养基(GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。
生长特性 贴壁生长
RAG小鼠肾腺癌细胞详细说明
动物种别 小鼠
描述 这是一株肾脏-2α细胞株的非回复8-氮杂鸟嘌呤抗性(HPRT-, HGPRT-)克隆。 测试发现缺肢畸形病毒(鼠痘)阴性。 在本库通过支原体检测。
一、产品信息
| Organism | Mus musculus, mouse |
|---|---|
| Tissue | kidney |
| Product Format | frozen |
| Morphology | amoeboid |
| Culture Properties | adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | renal adenocarcinoma |
| Strain | BALB/c |
| Storage Conditions | liquid nitrogen vapor phase |
二、细胞特点
DerivationThis is a non-reverting 8-azaguanine resistant (HPRT -, HGPRT -) clone of the Renal-2a cell line.Genes Expressed
三、培养方法
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle＇s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Note: For more information on enzymatic dissociation ａnd subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation | culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
|
| Culture Conditions | Temperature: 37°C |
四、发展历史
Name of DepositorRJ Klebe, FH RuddleReferences
Klebe RJ, et al. Mapping of a human genetic regulator element by somatic cell genetic analysis. Proc. Natl. Acad. Sci. USA 66: 1220-1227, 1970. PubMed: 4920091
Klebe RJ, et al. Controlled production of proliferating somatic cell hybrids. J. Cell Biol. 45: 74-82, 1970. PubMed: 4318843
Felluga B, et al. Electron microscope observations on virus particles associated with a transplantable renal adenocarcinoma in BALB-cf-Cd mice. J. Natl. Cancer Inst. 43: 319-333, 1969. PubMed: 5797837
Hay, R. J., Caputo, J. L., ａnd Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. ａnd Vesley, D., (1995) Laboratory Safety: Principles ａnd Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological ａnd Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health ａnd Human Services, Centers for Disease Control ａnd Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is availableonline.
