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NCTC clone 929 小鼠成纤维细胞

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价格：￥电议

更新日期：2026/9/8 3:56:46

生产地：美洲

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简单介绍：上海享世公司和ATCC共同努力致力于为中国大陆和澳门地区的客户提供可靠的研究材料以及最方便、最快捷、最有保障的服务。复苏细胞：将含有 1mL 细胞悬液的冻存管在 37℃水浴中迅速摇晃解冻，加入 4mL 培养基混合均匀。在 1000RPM 条件下离心 4 分钟，弃去上清液，补加 1-2mL 培养基后吹匀。然后将所有细胞悬液加入培养瓶中培养过夜（或将细胞悬液加入 10cm 皿中

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相关标签：NCTC,clone,929,小鼠成纤维细胞,NCTC,小鼠成纤维细胞。ATCC小鼠成纤维细胞,

详细内容

一、细胞信息

Organism	Mus musculus, mouse
Tissue	subcutaneous connective tissue; areolar ａnd adipose
Product Format	frozen
Morphology	fibroblast
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age	100 days
Gender	male
Strain	C3H/An
Applications	This cell line can be used for toxicity testing. This cell line is a suitable transfection host.
Storage Conditions	liquid nitrogen vapor phase

二、细胞特点

Karyotype	modal chromosome number = 66; range = 65 to 68. There were approximately 20 to 30 marker chromosomes present in each metaphase spread. A high percentage of those markers were common to most analyzed cells. A long metacentric chromosome with secondary constriction was noted in 77/100 cells. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Images
Derivation	The parent L strain was derived from normal subcutaneous areolar ａnd adipose tissue of a 100-day-old male C3H/An mouse. NCTC clone 929 (Connective tissue, mouse) Clone of strain L was derived in March, 1948. Strain L was one of the first cell strains to be established in continuous culture, ａnd clone 929 was the first cloned strain developed. Clone 929 was established (by the capillary technique for single cell isolation) from the 95th subculture generation of the parent strain.
Clinical Data	male mouse 100 days
Antigen Expression
Tumorigenic	Yes, in immunosuppressed mice
Effects	Yes, in immunosuppressed mice
Virus Susceptibility	Vesicular stomatitis, Glasgow (Indiana) Vesicular stomatitis, Orsay (Indiana) Encephalomyocarditis virus
Virus Resistance	poliovirus 1, 2, 3; coxsackievirus B5; polyomavirus
Comments	This cell line has been tested ａnd found negative for ectromelia virus (mousepox).

三、培养方法

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Eagle＇s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing	Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T⁻⁷5 flasks (catalog #430641) are recommended for subculturing this product. Remove ａnd discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask ａnd observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium ａnd aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: Air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C

四、发展历史

Name of Depositor

WR Earle

Year of Origin

March, 1948

References

Kazazian HH Jr., et al. Restriction site polymorphism in the phosphoglycerate kinase gene on the X chromosome. Hum. Genet. 66: 217-219, 1984. PubMed: 6325324

Fisher EM, et al. Homologous ribosomal protein genes on the human X ａnd Y chromosomes: escape from X inactivation ａnd possible implications for Turner syndrome. Cell 63: 1205-1218, 1990. PubMed: 2124517

Sanford KK, et al. The growth in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 9: 229-246, 1948.

Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal ａnd transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111

ASTM International Standard Practice for Direct Contact Cell Culture Evaluation of Materials for Medical Devices. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 0813-07.

ASTM International Standard Test Method for Agar Diffusion Cell Culture Screening for Cytotoxicity. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 0895-84 (Reapproved 2006).

Westfall BB, et al. The glycogen content of cell suspensions prepared from massive tissue culture: comparison of cells derived from mouse connective tissue ａnd mouse liver. J. Natl. Cancer Inst. 14: 655-664, 1953. PubMed: 13233820

Earle WR, et al. Production of malignancy in vitro. IV. The mouse fibroblast cultures ａnd changes seen in the living cells. J. Natl. Cancer Inst. 4: 165-212, 1943.

Earle WR, et al. The influence of inoculum size on proliferation in tissue cultures. J. Natl. Cancer Inst. 12: 133-153, 1951. PubMed:14874126

Sanford KK, et al. The tumor-producing capacity of strain L mouse cells after 10 years in vitro. Cancer Res. 16: 162-166, 1956. PubMed: 13293658

Westfall BB, et al. The arginase ａnd rhodanese activities of certain cell strains after long cultivation in vitro. J. Biophys. Biochem. Cytol. 4: 567-570, 1958. PubMed: 13587550

Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991

Yasin B, et al. Susceptibility of Chlamydia trachomatis to protegrins ａnd defensins. Infect. Immun. 64: 709⁻⁷13, 1996. PubMed: 8641770

Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS ISO 10993.5-2002.

Plastics collapsible containers for human blood ａnd blood components－－Part 1: Conventional containers. Annex C. Biological tests.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 3826-1:2003.

Dentistry－－Preclinical evaluation of biocompatibility of medical devices used in dentistry－－Test methods for dental materials. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 7405:1997.

Biological evaluation of medical devices－－Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10993-5:1999.

Plastic containers for intravenous injection. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 15747:2003.

ATCC CCL-1(NCTC clone 929)小鼠成纤维细胞接受后处理

1) 收到细胞后，请检查是否漏液，如果漏液，请拍照片发给我们。
2) 请先在显微镜下确认细胞生长状态，去掉封口膜并将T25瓶置于37℃培养约2-3h。
3) 弃去T25瓶中的培养基，添加 6ml本公司附带的完全培养基。
4) 如果细胞密度达80%-90%请及时进行细胞传代，传代培养用6ml本公司附带的完全培养基。

NCTC,clone,929,小鼠成纤维细胞,NCTC,小鼠成纤维细胞。ATCC小鼠成纤维细胞,

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