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NG108-15细胞株
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价格：￥电议
更新日期：2026/9/8 3:56:46
生 产 地：美洲
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详细内容
细胞生长：贴壁生长或悬浮生长
细胞形态：上皮样、多形态细胞样等形态
细胞数量：1×1000000个细胞数
细胞纯度：93％
NG108-15细胞，小鼠神经母细胞瘤细胞与大鼠胶质瘤细胞之融合细胞活力：87％（Viability by Trypan Blue Exclusion）
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌
细胞运输：干冰运输（1 Vial）或活细胞运输（T-25 flasks）
细胞用途：只可用于科研，不可用于临床诊断和治疗
NG108-15细胞，小鼠神经母细胞瘤细胞与大鼠胶质瘤细胞之融合细胞培养的注意事项：
玻璃吸管和玻璃培养瓶的消毒：1)高压蒸汽灭菌15分钟以上；2)干烤消毒140度2小时以上；无菌工作台先清洗后用75%酒精擦拭干净，紫外线照射40分钟以上；各种培养板照射3小时以上；培养基(pH7.2)和血清配制好后要做无菌试验:将血清按10%加入培养基内，用无菌的玻璃离心管或玻璃瓶取完全培养基5-10ml置培养箱内培养2-3天，肉眼见无浑浊或沉淀等异物。分装后置4度保存；消化液(pH7.8)或其它加入液，应用高压蒸汽灭菌或一次性无菌滤器过滤除菌，分装成支置-20度保存；培养箱应先用清水清洗后用75%酒精擦拭一遍(如有紫外线的应照射1小时以上，如有高温灭菌的应按程序来菌)。至少每月一次。
进入操作时应注意先清洗双手及手腕，然后用75%酒精擦拭，操作时注意无菌台内空间层次。手及物品不要在暴露的瓶口上方来往，如果数量较多，培养瓶应放在与酒精灯平行地方便于操作，瓶与瓶之间应相隔一定的距离，开瓶(盖)时应先用75%酒精反复擦拭或用灯烧，打开后应用灯先烧口，然后烧盖。用完后同样操作。整个操作过程尽量在无菌台靠里面一点。
一、细胞信息
| Organism | Mus musculus (neuroblastoma); Rattus norvegicus (glioma), mouse (neuroblastoma); rat (glioma) |
|---|---|
| Tissue | brain |
| Cell Type | somatic cell hybrid |
| Product Format | frozen |
| Morphology | flat; round; 10 to 100 micrometers diameter |
| Culture Properties | Adherent, but please note: as the culture media becomes acidic these cells begin to detach ａnd grow as a suspension, but they will typically reattach again when fresh medium is added. |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | glioblastoma; neuroblastoma |
| Applications | This cell line is a suitable transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Disclosure | This material is cited in a US or other Patent ａnd may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
二、细胞特点
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三、培养方法
| Complete Growth Medium | The base medium for this cell line is Dulbecco＇s Modified Eagle＇s Medium (GIBCO/InVitrogen Catalog No.12100-061, DMEMwithout sodium pyruvate ). To make the complete growth medium, add the following components to the base medium:
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|---|---|
| Subculturing | Remove medium, ａnd rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution ａnd add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate ａnd dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
