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F9小鼠畸胎瘤细胞ATCC上海细胞库
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价格：￥电议
更新日期：2026/9/8 3:56:46
生 产 地：美洲
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详细内容
| Organism | Mus musculus, mouse |
|---|---|
| Tissue | testis |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | embryonal carcinoma; testicular teratoma |
| Age | embryo |
| Strain | 129 |
| Applications | This cell line is a suitable transfection host. |
| Genes Expressed | plasminogen activator; laminin; type IV collagen |
|---|---|
| Cellular Products | plasminogen activator; laminin; type IV collagen |
| Comments | F9 cells can be stimulated to differentiate into parietal endoderm in the presence of retinoic acid ａnd dibutyryl cyclic AMP (cAMP). Differentiating cells synthesize plasminogen activator, laminin ａnd type IV collagen. cAMP is active only on cells that have been treated with retinoic acid. The cells maintain three copies of the beta 1 integrin gene. Tested ａnd found negative for ectromelia virus (mousepox). |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco＇s Modified Eagle＇s Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. NOTE: culture vessels must be coated with 0.1% gelatin prior to use. To do so, cover the surface of the vessel with 0.1% gelatin from porcine skin (Sigma G2500 or G1890) in sterile distilled water for 2 hours at 4°C, then wash three times with sterile distilled water. Treated flasks ａnd dishes can be stored at room temperature.
Subcultivation Ratio: A subcultivation ratio of 1:10 is recommended Medium Renewal: Every 2 to 3 days |
| Culture Conditions | Temperature: 37°C |
| Name of Depositor | S Strickland |
|---|---|
| References | Strickland S, et al. Hormonal induction of differentiation in teratocarcinoma stem cells: generation of parietal endoderm by retinoic acid ａnd dibutyryl cAMP. Cell 21: 347-355, 1980. PubMed: 6250719 Strickland S, Mahdavi V. The induction of differentiation in teratocarcinoma stem cells by retinoic acid. Cell 15: 393-403, 1978. PubMed: 214238 Stephens LE, et al. Targeted deletion of beta 1 integrins in F9 embryonal carcinoma cells affects morphological differentiation but not tissue-specific gene expression. J. Cell Biol. 123: 1607-1620, 1993. PubMed: 7504677 Berstine EG, et al. Alkaline phosphatase activity in mouse teratoma. Proc. Natl. Acad. Sci. USA 70: 3899-3903, 1973. PubMed: 4521215 Jang SI, et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649 |
F9细胞，小鼠畸胎瘤细胞
培养条件
DMEM-H10%FBS
细胞生长：F9细胞，小鼠畸胎瘤细胞贴壁生长
细胞形态：上皮样
细胞数量：1×106个细胞数
细胞传代：1:10传代2~3天1次
细胞特性：在维甲酸和联丁酰环磷腺苷CAMP）刺激下F9细胞分化成体壁内胚层。分化的细胞合成血浆酶原活化因子、层粘连蛋白和Ⅳ型胶原质。只有在经过维甲酸处理后细胞上的cAMP才有作用。细胞中有3个拷贝β1整合素基因。检测表明鼠痘病毒阴性
细胞纯度：92％
我们有细胞库和专业实验室无菌操作，同时接受客户复苏、委托培养服务，并提供F9细胞，小鼠畸胎瘤细胞的科研项目解决方案以及实验服务。购买我司细胞的客户请先与公司细胞销售人员核实订购的细胞株名称、种属、货号、数量、协商细胞价格并预约发货期与提取方式。向销售人员索取细胞株说明书并认真阅读以方便你的实验操作。
对于F9细胞，小鼠畸胎瘤细胞培养，只要我们细心操作，注意细节，并不是大家说的那样困难。对于有经验和有条件的客户，我们提供专人指导。对于无培养经验和条件的客户，建议由公司代为培养细胞及进行后续研究。我司对每一位客户追踪服务，因材施教，对产品不仅售前负责，售后更负责。
