文章标题：Epigenetic Silencing of GPD1 by HDAC2 via H3K9 Deacetylation Promotes Head and Neck Squamous Cell Carcinoma Progression

作者列表：Xiang Li, Qing Wang, Jiecheng Zhang, Wenjun Zhao, Xinting Zheng

影响因子：3.5

期刊：EXPERIMENTAL CELL RESEARCH

发表时间：2026-4-8

DOI:10.1016/j.yexcr.2026.115018

文献主题：Abstract

Background

Head and neck squamous cell carcinoma (HNSCC) remains a lethal malignancy with its pathogenic mechanisms incompletely unraveled. This study interrogates the role of the HDAC2-GPD1 axis in driving HNSCC progression.

Methods

HDAC2 and GPD1 expression was analyzed in HNSCC tissues (IHC) and cell lines (RT-qPCR). TCGA-HNSCC dataset (520 tumors, 44 normal controls) was used for bioinｆormatics analysis of gene expression, correlation, and prognostic value. Stable HNSCC cell lines with HDAC2/GPD1 overexpression or knockout were established via lentiviral infection. CCK-8, Transwell, wound healing, and flow cytometry were perｆormed to assess cell proliferation, invasion, migration and apoptosis in vitro. ChIP assays were conducted to verify the binding of HDAC2 to the GPD1 promoter and the H3K9ac modification of GPD1. Nude mice were implanted with modified FaDu cells to assess tumor growth via tumor measurements and HE analyses.