名称人肝癌细胞SNU-387鉴定
别称 SNU387; NCI-SNU-387
种属 人
年龄（性别） 女；41岁
组织来源 肝癌
生长特性 贴壁细胞
细胞形态 上皮细胞样
背景描述
SNU-387 was derived in 1990 by J.-G. Park ａnd associates from a primary hepatocellular carcinoma ta
a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus a com
of doxorubicin ａnd mitomycin-C. Tumor cells were initially cultured in ACL-4 medium supplemented
heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 sup
with 10% heat inactivated fetal bovine serum.
生物安全等 2
生长培养基 RPMI-1640(PM150110)＋10% FBS(164210-500)＋1% P/S(PB180120)
推荐传代比例 1:3-1:6
推荐换液频率 2~3次/周
倍增时间 61 hours (PubMed=7543080)
冻存条件 冻存液：55% 基础培养基+40%FBS+5%DMSO
温度：液氮
培养条件 气相：空气，95%；CO2，5%
温度：37℃
保藏机构 ATCC; CRL-2237
名称人肝癌细胞SNU-387鉴定
别称 SNU387; NCI-SNU-387
种属 人
年龄（性别） 女；41岁
组织来源 肝癌
生长特性 贴壁细胞
细胞形态 上皮细胞样
背景描述
SNU-387 was derived in 1990 by J.-G. Park ａnd associates from a primary hepatocellular carcinoma ta
a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus a com
of doxorubicin ａnd mitomycin-C. Tumor cells were initially cultured in ACL-4 medium supplemented
heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 sup
with 10% heat inactivated fetal bovine serum.
生物安全等 2
生长培养基 RPMI-1640(PM150110)＋10% FBS(164210-500)＋1% P/S(PB180120)
推荐传代比例 1:3-1:6
推荐换液频率 2~3次/周
倍增时间 61 hours (PubMed=7543080)
冻存条件 冻存液：55% 基础培养基+40%FBS+5%DMSO
温度：液氮
培养条件 气相：空气，95%；CO2，5%
温度：37℃
保藏机构 ATCC; CRL-2237
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