【文献标题】Anti-inflammation Effects of Cordyceps sinensis Mycelium in Focal Cerebral Ischemic Injury Rats 【作者】Zhenquan Liu,Pengtao Li,Dan Zhao,et.al 【作者单位】北京中医药大学（ Beijing University of Chinese Medicine） 【文献中引用产品】 大鼠白介素1β (IL-1β)elisa试剂盒 大鼠肿瘤坏死因子α（TNF-α）ELISA试剂盒 【关键词】CSM; inflammation; ischemia–reperfusion; inflammatory cells; inflammatory mediators. 【DOI】https://10.1007/s10753-010-9273-5 【影响因子(IF)】5.5 【出版期刊】《Inflammation》 【产品原文引用】 Measurement of IL-1β ａnd TNF-α Level Protein concentration of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured by the way introduced by Z. Cai [20]. Briefly, rats were killed after grip neurobehavioral test. Protein was extracted from the whole brain ａnd the concentration was adjusted to 4 mg/ml. The brain concentration of IL-1β, TNF-α was then determined using a commercial enzyme-linked immunosorbent assay (ELISA) kit (Shanghai Jinma Biological Technology, Inc., China) following the manufacture’s instruction.
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