人叉头框C2(FOXC2)elisa试剂盒2022年价格_产品说明_仪器仪表技术文献

人叉头框C2(FOXC2)elisa试剂盒2022年价格

提供者：上海极威生物科技有限公司发布时间：2022/11/16 16:58:19 阅读次数：3008次进入该公司店铺

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人叉头框C2（FOXC2）ELISA检测试剂盒

使用说明书

检测原理

试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被人叉头框C2（FOXC2）捕获抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻di洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的人叉头框C2（FOXC2）呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。

试剂盒组成

名称	96孔配置	48孔配置	备注
微孔酶标板	12孔×8条	12孔×4条	无
标准品	0.3mL	0.3mL	无
样本稀释液	6mL	3mL	无
检测抗体-HRP	10mL	5mL	无
20×洗涤缓冲液	25mL	15mL	按说明书进行稀释
底物A	6mL	3mL	无
底物B	6mL	3mL	无
终止液	6mL	3mL	无
封板膜	2张	2张	无
说明书	1份	1份	无
自封袋	1个	1个	无

注：标准品浓度依次为：1200、600、300、150、75、0 pg/mL.

试剂的准备

20×洗涤缓冲液的稀释：蒸馏水按1：20稀释，即1份的20×洗涤缓冲液加19份的蒸馏水。

HumanFOXC2(FOXC2) ELISA Kit instruction

Intended use

This FOXC2 ELISA kit is intended Laboratory for Research use only ａnd is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow ａnd the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of FOXC2 in the sample, this FOXC2 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples ａnd allow the operator to produce a standard curve of Optical Density versus FOXC2 concentration. The concentration of FOXC2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials supplied

Name	96determinations	48determinations
Microelisa stripplate	12*8strips	12*4strips
Standard	0.3ml	0.3ml
Sample diluent	6.0ml	3.0ml
HRP-Conjugate reagent	10.0ml	5.0ml
20X Wash solution	25ml	15ml
Chromogen Solution A	6.0ml	3.0ml
Chromogen Solution B	6.0ml	3.0ml
Stop Solution	6.0ml	3.0ml
Closure plate membrane	2	2
User manual	1	1
Sealed bags	1	1

Note: Standard concentration was followed by:

1200、600、300、150、75、0 pg/mL.

Reagent preparation

20×wash solution:Dilute with Distilled or deionized water1:20.

Assay procedure

1. Prepare allreagentsbeforestartingassayprocedure. ItisrecommendedthatallStandardsand Samplesbe addedin duplicateto the MicroelisaStripplate.

2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.

3. Add Sample: Add testing sample10μl Then add sample diluent 40μl to testing sample well; Blank welldoesn’t add anyting.

4. Add100μlofHRP-conjugate reagentto each well,cover with an adhesive stripandincubatefor60 minutes at37°C.

5. Aspirate each well ａnd wash, repeating the process four times for a total of five washes.Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifolddispenseror autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating ordecanting. Invert the plate ａnd blot it against clean paper towels.

6. Add chromogen solution A 50μl ａnd chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.

7. Add 50μl Stop Solution to each well. The color in the wells should change from blue toyellow. If the color in the wells is green or the color change does not

appear uniform,gently tap the plate to ensure thorough mixing.

8. ReadtheOpticalDensity(O.D.)at450nmusinga microtiterplatereaderwithin15minutes.