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技术文章
RGDSP功能化肽水凝胶通过整合素αv/PI3K/AKT轴刺激生长因子分泌，促进人羊膜间充质干细胞创面愈合
点击次数：2719 发布时间：2025/1/27 20:54:43
2024年10月，贵州省细胞工程重点实验室，遵义医学院附属医院,遵义医科大学附属医院临床干细胞研究所，湖北医科大学附属太和医院创面修复与皮肤外科(Key Laboratory of Cell Engineering of Guizhou Province, Affiliated Hospital of Zunyi Medical University, Zunyi, China,The Clinical Stem Cell Research Institute, Affiliated Hospital of Zunyi Medical University, Zunyi, China,Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, China)Wei Wei老师研究团队在《Frontiers in Bioengineering and Biotechnology》上发表论文：
“RGDSP-functionalized peptide hydrogel stimulates growth factor secretion via integrinαv/PI3K/AKT axis for improved wound healing by human amniotic mesenchymal stem cells”
“RGDSP功能化肽水凝胶通过整合素αv/PI3K/AKT轴刺激生长因子分泌，促进人羊膜间充质干细胞创面愈合”
Abstract：
The wound healing process involves communication among growth factors, cytokines, signaling pathways, and cells in the extracellular matrix, with growth factors acting as key regulators. Although stem cells can promote wound healing by secreting diverse growth factors, their therapeutic potential is hindered by poor survival and engraftment. Mimicking the stem cell-matrix interactions can improve stem cell survival, regulate their fate, and even enhance their paracrine effects. This study investigated the use of composite RGDmix hydrogel, which can support the survival and proliferation of human amniotic mesenchymal stem cells (hAMSCs), and effectively increase the expression of various growth factors, thereby promoting wound re-epithelialization, angiogenesis, and epidermal maturation. At last, the specific role of integrin αv and PI3K/AKT signaling pathways in the secretion of growth factors were examined by silencing them in vitro and in vivo. Results suggested that the RGDmix hydrogel improved the secretion of growth factors by hAMSCs through the RGDSP/integrin αv/PI3K/AKT axis, thereby enhancing the therapeutic effect in wound healing.
摘要：
伤口愈合过程涉及生长因子、细胞因子、信号通路和细胞外基质细胞之间的交流，其中生长因子是关键的调节因子。虽然干细胞可以通过分泌多种生长因子促进伤口愈合，但其治疗潜力受到生存和植入性差的阻碍。模拟干细胞与基质的相互作用可以提高干细胞的存活率，调节其命运，甚至增强其旁分泌作用。本研究研究了复合RGDmix水凝胶能够支持人羊膜间充质干细胞（hAMSCs）的存活和增殖，并有效增加各种生长因子的表达，从而促进创面再上皮化、血管生成和表皮成熟。，通过体外和体内实验，研究整合素αv和PI3K/AKT信号通路在生长因子分泌中的具体作用。结果表明，RGDmix水凝胶通过RGDSP/integrinαv/PI3K/AKT轴促进hAMSCs分泌生长因子，从而增强伤口愈合的治疗效果。
该论文中，hAMSCs的体外培养是使用Ausbian特级胎牛血清完成的。
原创作者：北京缔一生物科技有限公司
