Ⅰ、【基本介绍】

中文名称：ATTO 700-反式环辛烯；AT700-反式环辛烯

英文名称：ATTO 700 TCO；ATTO 700-TCO；AT700 TCO；AT700-TCO

外观：固体

分子量：874.44

荧光激发波长：700 nm

荧光发射波长：720 nm

溶解性：可溶于 DMSO，部分水溶

化学特性：TCO 活性，可与叠氮化物 click 化学反应

商家：西安强化生物科技有限公司

结构式：

纯度：≥95%

保存条件：避光，干燥，-20℃保存

操作规范

实验防护：操作时需穿戴一次性实验服及手套，避免直接接触试剂，确保实验人员安全。

温馨提示：该试剂只用于科研

Ⅱ、【试剂的相关阐述】

ATTO 700 is a near-infrared fluorescent dye characterized by strong absorbance, high photostability and thermal stability, and excellent water solubility. It is optimally excited in the 670-715 nm wavelength range.

译：ATTO 700是一种近红外荧光染料，其特点是吸收性强，光稳定性和热稳定性高，水溶性好。它在670-715 nm波长范围内被最佳激发。

As a zwitterionic compound, ATTO 700 maintains electrical neutrality when conjugated with biomolecules or other substrates. Its strong electron-accepting properties lead to effective fluorescence quenching of electron donors such as guanine and tryptophan. These characteristics make ATTO 700 an ideal choice for precise applications, including single-molecule detection and super-resolution microscopy techniques such as PALM, dSTORM, and STED. Additionally, ATTO 700 is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other biological assays, making it a versatile tool for advanced fluorescence research.

译：作为两性离子化合物，ATTO 700在与生物分子或其他底物结合时保持电中性。它的强电子接受特性导致了鸟嘌呤和色氨酸等电子供体的有效荧光猝灭。这些特性使ATTO 700成为精确应用的理想选择，包括单分子检测和超分辨率显微镜技术，如PALM， dSTORM和STED。此外，ATTO 700兼容流式细胞术（FACS）、荧光原位杂交（FISH）和各种其他生物检测，使其成为荧光研究的通用工具。

ATTO 700 TCO is particularly suitable for labeling tetrazine-modified biomolecules under copper-free conditions. It reacts with tetrazine-functionalized molecules to ｆorm stable conjugates through the dihydropyrazine moiety.

译：ATTO 700 TCO在无铜条件下特别适用于标记四嗪修饰的生物分子。它与四氮官能化的分子反应，通过二氢吡嗪部分形成稳定的共轭物。

Ⅲ、【相关试剂】

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本试剂由西安强化生物科技有限公司XAJ精心整理！本试剂只用于科研用途，特此声明！！！