Mouse Anti-Collagen II
Catalog Number : Rs-0709R
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: Collagens are highly conserved throughout evolution ａnd are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some antibodies with denatured collagen or formalin fixed, paraffin embedded tissues. Type II collagen is a fibrillar collagen found in cartilage ａnd the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth ａnd for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis,chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, ａnd spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
Specificity : · anti- Collagen II is a Mouse polyclonal antibody, unconjugated
Unconjugated
· specific for Collagen II of hu, rat, sheep, dog, bovine
· epitope mapping in the middle of Collagen II of hu, rat, sheep, dog, bovine
· Protein G affinity chromatography purification, purity :>96%
· Isotype: IgG
· use for western blotting, elisa, immunoprecipitation ａnd
immunohistochemistry
· mol wt: 161kDa
Mouse Anti-Collagen II
Application: · Western blotting 1:100-500
· Immunohistochemistry 1:100-500
· ELISA 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Mouse Anti-Collagen II
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