清华大学化学肿瘤基因组学实验室使用英克胎牛血清发表已见刊2025已更新

清华大学化学肿瘤基因组学国家重点实验室使用Invigentech胎牛血清发表文章到Cell Reports Medicine已见刊。

一、2020第四季度，清华大学化学肿瘤基因组学国家重点实验室发表标题为：An Automated Organoid Platform with Inter-organoid Homogeneityａnd Inter-patient Heterogeneity的文章到Cell Reports Medicine；

二、使用invigentech（英克)A6903FBS-500,FBS Uruguay Origin胎牛血清对小鼠肺，肝，肾，胰腺，脾脏， 心脏等细胞进行培养；

三、文章部分内容见下：

Mouse tissue processing

BALB/c mice (male, 6 - 8-weeks old) were euthanized by excessive CO2. Then, the organs (lung, liver, kidney, pancreas, spleen,heart) were isolatedａnd cut into small pieces (1 - 3 mm3 for each piece). After being washed with cold PBS (1 3 ) for three timesａnd supplemented with 2.0% penicillin-streptomycin (vol/vol, GIBCO), the tissues were digested by 1.0 mg/mL collagenase type I (Sigma-Aldrich)ａnd 2.0% fetal bovine serum (FBS) (vol/vol, Invigentech) on an orbital shaker at 37Cａnd incubated for 1 - 2 h. After digestion, the tissues were sheared by 10 mL plastic pipettes. The suspension was passed through a 100 mm filter (Falcon),ａnd centrifuged at 1000 rpm for 5 min. The pellet was resuspended in 5 mL red blood cell lysis buffer (Roche) for 5 min at room temperature. Afterward, the suspension was recentrifuged at 1000 rpm for 5 min,ａnd the pellet was resuspended in DMEM/F12 medium.The cell number was counted for organoid fabrication.