Sample : 2 μL
(Blank)Tween 20 47 mg/L (H₂O)
(Model solution)
Tween 20 47 mg/L + IgG 1 g/L
(in 0.3 M NaCl aq.)

Sample : 2 μL
(Blank)Tween 80 57 mg/L (H₂O)
(Model solution)
Tween 80 57 mg/L + IgG 1 g/L
(in 0.3 M NaCl aq.)

Column : Shodex ODP2 HP-2D (2.0 mm I.D. x 150 mm)

Eluent : (A) 0.1 % NH3 aq. / (B) CH3CN

High pressure linear gradient;

(B %) 20 %(0 to 5 min), 20 to 90 %(5 to 19 min), 90 %(19 to 24 min),

90 to 20 %(24 to 25 min)

Flow rate : 0.2 mL/min

Detector : UV (280 nm), ESI-MS (SIM Positive)

Column temp. : 40 ℃