提供者：上海笃玛生物科技有限公司 发布时间：2024/8/30 14:08:50 阅读次数：2725次 进入该公司店铺
| Product Name | GRP78 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Synthetic peptide made to an internal portion of the human protein (within residues 250-300). [Swiss-Prot# P11021] |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFWB |
Recommended Starting Dilutions: For WB: Use at a concentration of 0.5 ug/ml. For FACS: Use at a dilution of 1:150. For ICC/IF: Use at a dilution of 1:50. Optimal dilutions/concentrations should be determined by the end user.: | |
| Species Reactivity | HumanMouseChickenRatSheep |
| Predicted Reactivity | Bovine, C. elegans, Drosophila, Primate, ZebrafishPrimate (100%), Bovine (100%), Zebrafish (100%), Drosophila (100%), C. elegans (100%) |
| Concentration | 1 mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | More |
|---|---|
| Target | HSPA5 |
| Tissue Specificity | Grp78 |
| Cellular Localization | Endoplasmic reticulum lumen. Melanosome. Note= Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
Simple Western: GRP78/HSPA5 Antibody [ - Simple Western lane view shows a specific band for GRP78 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Detection of Bip/Grp78 on HeLa whole cell extracts using
| Application Notes | Recommended Starting Dilutions: For WB: Use at a concentration of 0.5 ug/ml. For FACS: Use at a dilution of 1:150. For ICC/IF: Use at a dilution of 1:50. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4C short term. Aliquot ａnd store at -20C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, 30% glycerol ａnd 0.1% sodium azide was added as a preservative. |
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