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PI3 kinase p85 alpha antibody Catalog Number:OM268484

提供者:上海笃玛生物科技有限公司   发布时间:2024/8/22 11:59:52  阅读次数:2707次  进入该公司店铺

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Product Profile

Product NamePI3 kinase p85 alpha antibody
Antibody TypePrimary Antibodies
Product description
Rabbit polyclonal to PI3 kinase p85 alphaBinds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p11 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
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Immunogen
KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y580 of human PIK3R1.

Key Feature

ClonalityPolyclonal
IsotypeIgG
Host SpeciesRabbit
Tested Applications

IHC-PWB

ELISA: Use at 1:1,000:
Species Reactivity

Human

Concentration0.2 mg/ml
PurificationAffinity purified

Target Information

Gene Synonyms
Phosphatidylinositol 3-kinase regulatory subunit alpha
PtdIns-3-kinase p85-alpha
PIK3R1
GRB1
Phosphatidylinositol3kinase regulatory subunit alpha
PI3-kinase p85-subunit alpha
PI3K
PI3kinase p85subunit alpha
PtdIns3kinase p85alpha
Phosphatidylinosi
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TargetPIK3R1
Molecular Weight(MW)83.598 kDa (note)

Application

  • Application

    Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PIK3R1-pY580 , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Application

    Western blot analysis of PIK3R1 (arrow) using rabbit polyclonal PIK3R1 Antibody (Y580) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R1 gene (Lane 2).

Application Notes
ELISA: Use at 1:1,000:

Additional Information

FormLiquid
Storage InstructionsStore at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Storage BufferPBS with 0.09% (W/V) sodium azide

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