CD31 antibody Catalog Number：OM244943_产品说明_仪器仪表技术文献

CD31 antibody Catalog Number：OM244943

提供者：上海笃玛生物科技有限公司发布时间：2024/8/20 13:47:39 阅读次数：2741次进入该公司店铺

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Product Profile

Product Name	CD31 antibody
Antibody Type	Primary Antibodies
Product description	Rabbit monoclonal antibody [EPR3094] to CD31The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, ａnd some types of T-cells, ａnd makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily ａnd is likely involved in leukocyte migration, angiogenesis, ａnd integrin activation. [provided by RefSeq, May 2010] More
Immunogen	Synthetic peptide corresponding to residues in the extracellular domain of human CD31

Key Feature

Clonality	Monoclonal
Isotype	IgG
Clone Number	EPR3094
Host Species	Rabbit
Tested Applications	FACSIHC-PIPWB
	For WB: Use at a dilution of 1:5000 - 1:20000. For IP: Use at a dilution of 1:20. For FACS: Use at a dilution of 1:50. For IHC-P: Use at a dilution of 1:250 - 1:500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. We suggest to program the pressure cooker to run for 30 seconds at 125° C, followed by 10 seconds at 90° C. Then let the slide cool down to room temperature (10 - 20 minutes): Hide
Species Reactivity	Human
Concentration	1 mg/ml

Target Information

Gene Synonyms	platelet/endothelial cell adhesion molecule 1 GPIIA＇ PECAM-1 PECAM1 CD31 endoCAM PECA1 CD31/EndoCAM Hide
Target	CD31
Molecular Weight(MW)	83 kDa (note)

Application

Application
IHC-P analysis of human muscle tissue using CD31/PECAM-1 antibody [EPR3094] at a dilution of 1:250.
Application
WB analysis of (1) THP-1 ａnd (2) Jurkat lysates (10 μg per lane) using CD31/PECAM-1 antibody [EPR3094] at a dilution of 1:20,000.

Application Notes	For WB: Use at a dilution of 1:5000 - 1:20000. For IP: Use at a dilution of 1:20. For FACS: Use at a dilution of 1:50. For IHC-P: Use at a dilution of 1:250 - 1:500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. We suggest to program the pressure cooker to run for 30 seconds at 125° C, followed by 10 seconds at 90° C. Then let the slide cool down to room temperature (10 - 20 minutes): Hide

Application Notes

For WB: Use at a dilution of 1:5000 - 1:20000. For IP: Use at a dilution of 1:20. For FACS: Use at a dilution of 1:50. For IHC-P: Use at a dilution of 1:250 - 1:500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. We suggest to program the pressure cooker to run for 30 seconds at 125° C, followed by 10 seconds at 90° C. Then let the slide cool down to room temperature (10 - 20 minutes):

Hide

Additional Information

Form	Liquid
Storage Instructions	Shipped at 4°C. Upon delivery aliquot ａnd store at -20°C. Avoid freeze / thaw cycles.
Storage Buffer	PBS containing 50% Glycerol, 0.05% BSA ａnd 0.01% Sodium azide.