提供者：上海笃玛生物科技有限公司 发布时间：2024/8/14 15:34:28 阅读次数：2663次 进入该公司店铺
| Product Name | Occludin |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Synthetic peptide within human Occludin aa 1-50 conjugated to Keyhole Limpet Haemocyanin (KLH). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
WB:1:500-2,000 Hide ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1 mg/mL. |
| Alternative Names | Hide |
|---|---|
| Molecular Weight(MW) | 59 kDa |
| Cellular Localization | Cell membrane. |
Fig1: Western blot analysis of Occludin on mouse kidney (1) ａnd human kidney (2) tissue lysates using anti-Occludin antibody at 1/1,000 dilution.
Fig2: ICC staining Occludin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Occludin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Occludin antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Occludin antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of HepG2 cells with Occludin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
| Positive Control | mouse kidney ａnd human kidney tissue lysates, HepG2, Hela, A549, human lung tissue, mouse lung tissue. |
|---|---|
| Application Notes | WB:1:500-2,000 Hide ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
① 凡本网注明"来源：阿仪网"的所有作品，版权均属于阿仪网，未经本网授权不得转载、摘编或利 用其它方式使用。已获本网授 权的作品，应在授权范围内使用，并注明"来源：阿仪网"。违者本网将追究相关法律责任。
② 本信息由注册会员：上海笃玛生物科技有限公司发布并且负责版权等法律责任。
版权声明