人消退素D2（RvD2）ELISA试剂盒的检测范围是多少？

让上海仁捷生物告诉您：

1. 检测范围：12.5 pg/mL–400 pg/mL。

2. 灵敏度：最低检测浓度小于1.0pg/mL。

3. 特异性：不与其它可溶性结构类似物交叉反应。

4. 重复性：板内变异系数小于10%，板间变异系数小于15%。

操作步骤

1.从室温平衡20min后的铝箔袋中取出所需板条，剩余板条用自封袋密封放回4℃。

2.设置标准品孔和样本孔，标准品孔各加不同浓度的标准品50μL；

3.样本孔中加入待测样本50μL；空白孔不加。

4.除空白孔外，标准品孔和样本孔中每孔加入辣根过氧化物酶（HRP）标记的检测抗体100μL，用封板膜封住反应孔，37℃水浴锅或恒温箱温育60min。

5.弃去液体，吸水纸上拍干，每孔加满洗涤液（350μL），静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板5次（也可用洗板机洗板）。

6.每孔加入底物A、B各50μL，37℃避光孵育15min。

7.每孔加入终止液50μL，15min内，在450nm波长处测定各孔的OD值。

ThisRvD2ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofRvD2in the sample, thisRvD2ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusRvD2concentration. The concentration ofRvD2in the samples is then determined by comparing the O.D. of the samples to the standard curve.