4、 Bradford法测定蛋白浓度不受绝大部分样品中的化学物质的影响。样品中β-巯基乙醇的浓度可高达1M，二硫苏糖醇的浓度可高达5mM。 但受略高浓度的去垢剂影响。需确保SDS低于0.01%，Triton X-100低于0.05%，Tween 20,60,80低于0.015%。含去垢剂的样品推荐使用 本公司生产的BCA蛋白浓度测定试剂盒（BL521A）。染色时间不能太长，否则活细胞会因染料积累而染成颜色，使检测结果偏低。
考马斯亮蓝G250溶液室温保存，配置好的蛋白标准液-20℃冻存。效期年。
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