【文献标题】Zearale-induced aberration in the composition of the gut microbiome ａnd function impacts the ovary reserve
【作者】Shaojing Tan , Wei Ge , Junjie Wang ,et.al
【作者单位】青岛农业大学（Qingdao Agricultural University）
【文献中引用产品】
小鼠缪勒管抑制物质/抗缪勒管激素(MIS/AMH)elisa试剂盒
小鼠雌二醇(E2)ELISA试剂盒
小鼠促卵泡生长激素（FSH）ELISA试剂盒
小鼠黄体生成素释放激素(LHRH)ELISA试剂盒
【关键词】Zearale，Gut microbiome，Lysophosphatidylcholines，Reproductive disorder
【DOI】https://doi.org/10.1016/j.chemosphere.2019.125493
【影响因子(IF)】5.77
【出版期刊】《Chemosphere》
【产品原文引用】
Enzyme linked immunosorbent assay
Plasma from each mice was collected to evaluate the relative content of anti-mullerian hormone (AMH), 17 b-estradiol (E2),follicle-stimulating hormone (FSH), ａnd luteinizing hormone (LH) using ELISA kit(Jinma, Shanghai, China) following the manufacturer’s instruction. Briefly, the standardy ａnd serum samples were added ａnd adsorbed to wells of an ELISA plate, the enzyme-labeled plate was covered with microplate sealers ａnd incubated at 37 C for 30 min. Then the wash buffer was used to wash plate for five times (30 s per time), 50 ml horseradish peroxides (HRP)-conjugated reagent was added into each well ａnd incubated at 37 C for 30 min, After that the plate was washed 5 times again, ａnd each well was added 50 ml TMB substrate A&B solution in turn ａnd incubated at 37 C for 10 min. Finally, equal volumes of s solution were added into each well, the absorbance was tested andcalculated in 15 min by Multiscan Spectrum (450 nm).

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