【文献标题】Effects of dietary supplementation of inulin on rumen fermentation ａnd bacterial microbiota, inflammatory response ａnd growth performance in finishing beef steers fed high or low-concentrate diet
【作者】Ke Tian,Junhui Liu,Yawang Sun,et.al
【作者单位】西南大学（Southwest University）
【文献中引用产品】
牛脂多糖结合蛋白(LBP)elisa试剂盒
牛结合珠蛋白/触珠蛋白(Hpt/HP)elisa试剂盒
牛血清淀粉样蛋白A(SAA)ELISA试剂盒
牛C反应蛋白(CRP)ELISA试剂盒
【关键词】Inulin,Rumen,Bacterial microbiota,Lipopolysaccharide,Beef steer
【DOI】https://doi.org/10.1016/j.anifeedsci.2019.114299
【影响因子(IF)】5.0
【出版期刊】《Animal Feed Science ａnd Technology》
【产品原文引用】
Plasma cytokines ａnd APPs assay
The bovine-exclusive ELISA kit was used to measure the concentrations of plasma IL-1β, IL-6, IL-8 ａnd TNF-α. The bovine ELISA kits were purchased from Shanghai Sinobest Biotechnology Co., Ltd. (Shanghai, China). APPs including LPS-binding protein (LBP),haptoglobin (Hp), serum amyloid A (SAA) ａnd C-reactive protein (CRP) were determined using the bovine ELISA kits purchased from Shanghai Jinma Biotechnology Co., Ltd. (Shanghai, China). The determination was conducted according to the manufacturer’s instructions. All samples including the standards were tested in duplicate, ａnd the optical density values were read on a microplate reader (Bio-Rad, xMarkTM, USA) at 450 nm