（Bromodeoxyuridine）
Cat. Number: Rsm-0917M

Quantity size: 0.2ml （dilute with pH 7.4 0.01 M PBS or diluent of antibody）

Background: Bromodeoxyuridine （BrdU） is a thymidine analog ａnd is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling ａnd detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, ａnd the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been used for identi-fying proliferating cells in blood （Campana et al., 1988）, tissues （Schutte et al., 1987; Hayashi, et al., 1988）, tumors （Hoshino et al., 1986; Morstyn et al., 1983）, as well as for determining plasma cell labeling indices （Greipp et al., 1985）.

Specificity:· anti-BrdU is a mouse monoclonal antibody unconjugated

· use for elisa, immunoprecipitation ａnd immunohistochemistry

·affinity chromatography purification, purity :>95%

· Isotype: IgG

Application: · Immunohistochemistry: 1:100-500

· ELISA: 1:500-1000

· Optimal working dilutions must be determined by the end user.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.