Eluent:

A: H₂O/1% HAc
B: MeOH/1% HAc

Gradient:

5-100% B, 0-30 min

Flow:

1 ml/min

Detection:

UV 280 nm

Temperature:

25 °C

Injection:

2 µl

Sample:

1: Phenol
2: 4-Nitrophenol
3: 2,4- Dinitrophenol
4: 2-Chlorophenol
5: 2-Nitrophenol
6: 2,4-Dimethylphenol
7: 4,6-Dinitro-o-Cresol
8: 2,4-Dichlorophenol
9: 4-Chloro-m-Cresol
10: 2,4,6-Trichlorophenol
11: Pentachlorophenol