Anti- Vimentin抗体说明书， FITC conjugated现货供应单抗多抗标记抗体

Catalog Number :i RSF-0272R
Quantity size : 100ug （2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10mg/ml BSA ａnd 0.1% Sodium azide. ）

Anti- Vimentin抗体说明书， FITC conjugated现货供应单抗多抗标记抗体

Background: Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus ａnd plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments ａnd activation of an ATP ａnd myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, ａnd subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas （of neural, muscle ａnd fibroblast origin） compared with carcinomas which are generally negative. Melanomas, lymphomas ａnd vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms ａnd malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin ａnd neurofilaments.

Specificity : • anti- Vimentin is a rabbit monoclonal antibody, FITC conjugated
• specific for Vimentin of Goat, mouse ａnd rat
• Protein G affinity chromatography purification, purity :>95%
• Isotype: IgG
• mol wt：95kDa

Anti- Vimentin抗体说明书， FITC conjugated现货供应单抗多抗标记抗体

Application : • FCM: 1:20-100
• Immunohistochemistry : 1:20-1:200
• Immunocytochemistry: 1:20-1:200
• Excitation spectrum: 490-495nm
• Emission spectrum: 520-530nm
• Optimal working dilutions must be determined by the end user.
• protect from light.

Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Anti- Vimentin抗体说明书， FITC conjugated现货供应单抗多抗标记抗体

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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