elisa试剂盒除了对检测物质有较高的敏感性和特异性外，还必须具有良好的稳定性。稳定性研究是试剂盒产品质量控制的一个重要内容，也是确定试剂盒保存条件和有效期的重要依据。ELISA检测试剂盒的热稳定性好不仅可以延长试剂盒的使用时限，降低试剂盒的储存和运输条件的要求，而且也是试剂盒在现场检测的必备条件；反之，若稳定性差，即使所用试剂的特异性和敏感度非常好，试剂盒本身也失去现场应用的价值。
影响试剂盒热稳定性的因素主要有包被抗原浓度和包被量、抗体质量和效价、贮存温度等。一般而言，存放时间越长，贮存温度越高，试剂有效成分生物活性下降的速度也越快[18-20]。由本研究结果可见，本课题组自行研制的AFB1酶联免疫试剂盒在4℃保存270d的各项指标均满足试剂盒测定的要求；在37℃实验条件下保存13d，AFB1酶联免疫试剂盒也可以满足测定的要求。
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