Anti-Protein A蛋白说明书 , HRP conjugated

FROM:RICBIO
Catalog Number :RS-0362R-HRP

Quantity size :100ug （1mg/ml Buffer = 0.01M TBS（pH7.4） with 1% BSA, 0.03% Proclin300 ａnd 50% Glycerol.）

background:
Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus （SPA）. SPA binds proteins from many of mammalian species, most notably IgGs, ａnd helps inhibit phagocytic engulfment ａnd acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization ａnd phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 ａnd IgG2 as well as mouse IgG2a ａnd IgG2b, whereas bind with moderate affinity to human IgM, IgA ａnd IgE as well as mouse IgG3 ａnd IgG1. SPA is often produced in E. coli ａnd is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology ａnd other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies.

Anti-Protein A蛋白说明书 , HRP

Specificity : · Rabbit Polyclonal IgG, affinity purified by Protein A

· Reacts with: Human,rat

· Protein G affinity chromatography purification, purity :>95%

· Isotype: IgG

· mol wt：40-60kDa.kDa

Application : WB=1:100-500 ELISA=1:500-1000 IHC-F=1:100-500 IF=1:100-500

Optimal working dilutions must bedetermined by the end user

Anti-Protein A蛋白说明书 , HRP

Storage:Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.