Features:

       High performance vectors with optimized sequence

       Protein yield up to 10 times enhanced compared to protein expression with basic plasmid

       Plasmids with established MCS

       Encoding C- or N-terminal His-tag for versatile purification £¨native, 6xHis-tag£©

Secretion with LipA or YocH signal peptide up to 9 fold increased

optimized high performance £¨hp£© vectors for Bacillus megaterium!

MoBiTec provides a wide range of useful vectors for the B. megaterium system that are adaptable to most applications £ánd protein purification methods. These include vectors carrying a secretion signal peptide sequence £ánd a 6xHis-tag.

With our new hp-vectors yields up to 10-fold higher compared to the basic plasmids are possible. All plasmids have established multiple cloning sites £¨MCS£© for versatile cloning. Furthermore we offer vectors encoding C- or N-terminal His-tags for easy purification. The protein secretion with LipA or YocH signal peptides is up to nine-fold increased. Induction of protein expression of all vectors is achieved by the tightly regulated £ánd efficiently inducible xylose operon.

Versatile system with a wide range of vectors

The B. megaterium expression system provides a versatile £ánd easy-to-handle tool for stable £ánd high-yield protein production, both small- £ánd large-scale.

B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall.

B. megaterium protoplasts specifically optimized for transformation

The protoplasts supplied by MoBiTec are from B. megaterium strain WH320, developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies.

For secretion vectors we offer protoplasts of strain MS941. Both strains are mutants of DSM319, where WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.

Order Information, shipping £ánd storage

Please click on the respective order # for further details.

order# description amount

BMEG02 Bacillus megaterium protoplasts ready for transformation £¨strain WH320;1£©

Material is sufficient for 4 transformations plus control experiment. 5 x 500 µl

BMEG50 Bacillus megaterium protoplasts, strain MS941 5 x 500 µl

BMEG31 pC-His1623hp shuttle vector, C-term. 6xHis-tag; lyophilized DNA 10 μg

BMEG32 pN-His-TEV1623hp shuttle vector, N-term. His-tag,TEV-site 10 μg

BMEG30 p3STOP1623hp, shuttle vector, lyophilized DNA 10 μg

BMEG33 pSPLipA-hp, shuttle vector with signal sequence, lypophilized DNA 10 μg

BMEG34 pSPYocH-hp, shuttle vector with signal sequence, lypophilized DNA 10 µg

shipped at RT, protoplasts shipped on dry ice

store lyophilized vectors at 4°C, reconstituted vectors at -20°C, protoplasts at -70°C

All vectors are E. coli / B. megaterium shuttle vectors

Please note that protoplasts of Bacillus megaterium have a limited shelf life of some months only, depending on the date of production. Therefore purchase £ánd use of protoplasts must be thoroughly planned.