hp-Vector表达系统megaterium芽孢杆菌
hp-Vector表达系统megaterium芽孢杆菌
Features:
High performance vectors with optimized sequence
Protein yield up to 10 times enhanced compared to protein expression with basic plasmid
Plasmids with established MCS
Encoding C- or N-terminal His-tag for versatile purification （native, 6xHis-tag）
Secretion with LipA or YocH signal peptide up to 9 fold increased
optimized high performance （hp） vectors for Bacillus megaterium!
MoBiTec provides a wide range of useful vectors for the B. megaterium system that are adaptable to most applications ａnd protein purification methods. These include vectors carrying a secretion signal peptide sequence ａnd a 6xHis-tag.
With our new hp-vectors yields up to 10-fold higher compared to the basic plasmids are possible. All plasmids have established multiple cloning sites （MCS） for versatile cloning. Furthermore we offer vectors encoding C- or N-terminal His-tags for easy purification. The protein secretion with LipA or YocH signal peptides is up to nine-fold increased. Induction of protein expression of all vectors is achieved by the tightly regulated ａnd efficiently inducible xylose operon.
Versatile system with a wide range of vectors
The B. megaterium expression system provides a versatile ａnd easy-to-handle tool for stable ａnd high-yield protein production, both small- ａnd large-scale.
B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall.
B. megaterium protoplasts specifically optimized for transformation
The protoplasts supplied by MoBiTec are from B. megaterium strain WH320, developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies.
For secretion vectors we offer protoplasts of strain MS941. Both strains are mutants of DSM319, where WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.
Order Information, shipping ａnd storage
Please click on the respective order # for further details.
order# description amount
BMEG02 Bacillus megaterium protoplasts ready for transformation （strain WH320;1）
Material is sufficient for 4 transformations plus control experiment. 5 x 500 µl
BMEG50 Bacillus megaterium protoplasts, strain MS941 5 x 500 µl
BMEG31 pC-His1623hp shuttle vector, C-term. 6xHis-tag; lyophilized DNA 10 μg
BMEG32 pN-His-TEV1623hp shuttle vector, N-term. His-tag,TEV-site 10 μg
BMEG30 p3STOP1623hp, shuttle vector, lyophilized DNA 10 μg
BMEG33 pSPLipA-hp, shuttle vector with signal sequence, lypophilized DNA 10 μg
BMEG34 pSPYocH-hp, shuttle vector with signal sequence, lypophilized DNA 10 µg
shipped at RT, protoplasts shipped on dry ice
store lyophilized vectors at 4°C, reconstituted vectors at -20°C, protoplasts at -70°C
All vectors are E. coli / B. megaterium shuttle vectors
Please note that protoplasts of Bacillus megaterium have a limited shelf life of some months only, depending on the date of production. Therefore purchase ａnd use of protoplasts must be thoroughly planned.
ActiveCount 60 / 90CActiveCount 60, 90C, 60 / 90C浮游菌采样仪
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3m novec-72de清洗液/3MFC40/3mFC3283
上皮型钙粘附分子抗体，Anti-CD144/VECD/VE Cadherin，一抗 价格优惠 上海现货
| ■其它名称： | VE-cadherin;cadherin-5;Cdh5;Vascular endothelial cadherin;7B4 antigen;CD144 antigen;VECD;V |
| ■交叉反应： | rat, mo |
| ■产品类型： | 一抗 |
| ■应用领域: | /肿瘤/心血管/免疫学/信号转导/细胞粘附分子/血管内皮细胞/上皮细胞/内皮细胞/细胞外基质 |
| ■蛋白分子： | predicted molecular weight: 85-115kDa |
| ■浓度值： | 1mg/1ml |
| ■物理性状： | Lyophilized or Liquid |
| ■免疫原： | KLH conjugated synthetic peptide derived from human CD144 C-terminus |
| ■亚型： | polyclonal |
| ■纯化方法： | affinity purified by Protein A |
| ■保存液： | 0.01M PBS, pH 7.4 with 10 mg/ml BSA ａnd 0.1% Sodium azide |
| ■应用技术： | //WB=1:100-500//ELISA=1:500-1000//IP=1:20-100//IHC-P=1:100-500//IHC-F=1:100-500//IF=1:100-500（石蜡切片需做抗原修复）/not yet tested in other applications./optimal dilutions/concentrations should be determined by the end user. |
| ■保存环境： | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
1mg/1ml 上皮型钙粘附分子抗体 Anti-CD144/VECD/VE Cadherin VE-cadherin;cadherin-5;Cdh5;Vascular endothelial cadherin;7B4 antigen;CD144 antigen;VECD;V的来源为Rabbit，其相关产品：免疫组化常用试剂免疫印迹常用试剂各种标记二抗查询其它抗体目录请点击本公司化工仪器网链接：http://www.chem17.com/st233816/erlist_675408.html上海田源专业销售上皮型钙粘附分子抗体产品,更多请点击查询！
优质批发 pGEM-T easy Vector (T载体) 价格
pGEM-T easy Vector （T载体） 1.2ug
土霉素 进口分装 10g 罗红霉素 进口分装 10g 托普霉素 进口分装 1g EDTA Na2 乙二胺四乙酸二钠 Amresco 100g EDTA Na2 乙二胺四乙酸二钠 Amresco 500g EDTA FREE ACLD 乙二胺四乙酸 Amresco 100g EDTA FREE ACLD 乙二胺四乙酸 Amresco 500g EGTA 乙二醇－双－（2－氨基乙基）四乙酸 Amresco 5g EGTA 乙二醇－双－（2－氨基乙基）四乙酸 Amresco 10g Ethidium Brmide 溴化乙锭 Amresco 100mg Ethidium Brmide 溴化乙锭 Amresco 1g goldview 自产 1ml Evans Blue 埃文斯蓝 Sigma 1g Eosin Y 伊红Y（醇溶） 进口分装 10g Eosin Y 伊红Y Amresco 10g Ethephon 乙烯利 国产 25g Fluorescein diacetate二乙酸荧光素 Sigma 1g Fibrinogen 纤维蛋白原 Sigma 100mg Fibrinogen 纤维蛋白原 Sigma 1g Forchior fennron 氯吡苯脲 进口分装 1g Ficoll 400 聚蔗糖400 Pharmacia 10g
全国销售热线： 客服热线：
瑞士Vector/伟拓SDC-H1风道湿度变送器
- 空调冷热系统风道的送回风湿度测量,通风系统的湿度测量.
- 可记录测量湿度的和值.
- 可选择配置显示
| 型号名称 | 型号代码 | 描述/选项 |
| SDC-H1-08 | 40-30 0060 | 探针长度77mm （3”） |
| SDC-H1-16 | 40-30 0061 | 探针长度157mm （6.2”） |
| 型号名称 | 型号代码 | 精度 [%rH] | 精度[K]@25°C （77°F） | 描述/选项 |
| AES-HT-A2 | 40-50 0031-2 | 2% | ± 0.5° | 温湿度敏感元件 |
| AES-HT-A3 | 40-50 0031-3 | 3% | ± 0.4° | |
| AES-HT-A5 | 40-50 0031-5 | 4.5% | ± 0.3° |
| 型号名称 | 型号代码 | 描述/选项 |
| OPC-S | 40-50 0029 | 罩壳显示 |
| OPA-S | 40-50 0006 | 外接显示 |
| AMC-1 | 20-10 0035 | 连接线缆Æ 4 – 8 mm （AWG 6 – 1） |
| AMC-2 | 20-10 0067 | 线缆连接头 NPT 1/2 |
32713EasyXpress pIX4.0 Vector
www.huaxiatech.cn
EasyXpress pIX4.0 Vector
Vector试剂
Vector试剂
A1360pGEM-T easy Vector (T载体）
详细介绍：产品名称：
| pGEM-T easy Vector （T载体） |
检测试剂：免疫检测、基因检测、化学检测
原料试剂：抗原抗体、分子生物、生化试剂北京雅杰宝生物科技有限公司“产品名称”新品上市，本公司主营产品：elisa试剂盒、生物试剂、血清、抗体、培养基、标准品，，，RD欢迎咨询。
pAT5 vector价格
pAT5 vector价格 规格：10ug × 5支，产品的价格|使用说明，全国配送方式
公司专业经销ELISA试剂盒多年,产品质量有,现货销售elisa试剂盒，培养基，标准品，抗体，生物试剂，生化试剂等试剂产品。供应现货产品，品质齐全，请来电咨询。
pAT5 vector价格 及相关产品库。
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