Anti-CDK7,周期素依赖性激酶7抗体

• Product nameAnti-Cdk7 antibody [MO1.1]See all Cdk7 primary antibodies ...
• Description
  Mouse monoclonal [MO1.1] to Cdk7
• SpecificityThis antibody is specific to human CAK （CDK-activating kinase）, a 40 kDa protein.
• Tested applicationsFlow Cyt, ICC/IF, IP, WB, IHC-Fr more details
• Species reactivity
  Reacts with: Mouse, Human
• Immunogen

  Recombinant 221 amino acid fragment of the C-terminus of human p40^M015.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term （1-2 weeks）. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• PurityTissue culture supernatant
• ClonalityMonoclonal
• Clone numberMO1.1
• Myelomaunknown
• IsotypeIgG2b
• Light chain typeunknown
• Research Areas
  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Cdks

  • Epigenetics ａnd Nuclear Signaling
  • Cell cycle
  • Kinases/Phosphatases
  • Cdks

Applications

Target

• FunctionCyclin-dependent kinases （CDKs） are activated by the binding to a cyclin ａnd mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase （CAK） complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 ａnd CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain （CTD） of its large subunit （POLR2A）, allowing its escape from the promoter ａnd elongation of the transcripts. Involved in cell cycle control ａnd in RNA transcription by RNA polymerase II. Its expression ａnd activity are constant throughout the cell cycle.
• Tissue specificityUbiquitous.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsPhosphorylation of Ser-164 during mitosis inactivates the enzyme.
  Phosphorylation of Thr-170 is required for activity.
• Cellular localizationNucleus.
• Information by UniProt
• Database links
  • Entrez Gene: 1022 Human
  • Entrez Gene: 12572 Mouse
  • Omim: 601955 Human
  • SwissProt: P50613 Human
  • SwissProt: Q03147 Mouse
  • Unigene: 184298 Human
  • Unigene: 259718 Mouse
• Alternative names
  • 39 kDa protein kinase antibody
  • CAK antibody
  • CAK1 antibody

  see all

Anti-Cdk7 antibody [MO1.1] images

• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Cdk7 antibody [MO1.1] （ab9516）
  ab9516 staining human cervical cancer cells by IHC-P.
• 

  Flow Cytometry-Anti-Cdk7 antibody [MO1.1]（ab9516）
  Overlay histogram showing HeLa cells stained with ab9516 （red line）. The cells were fixed with 80% methanol （5 min） ａnd then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody （ab59516, 1µg/1x10⁶ cells） for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG （H+L） （ab96879） at 1/500 dilution for 30 min at 22ºC. Isotype control antibody （black line） was mouse IgG2b [PLPV219] （ab91366, 2µg/1x10⁶ cells ） used under the same conditions. Acquisition of >5,000 events was performed.
  
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cdk7 antibody [MO1.1] （ab9516）
  ICC/IF image of ab9516 stained MCF7 cells. The cells were 4% formaldehyde （10 min） ａnd then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells ａnd block non-specific protein-protein interactions. The cells were then incubated with the antibody （ab9516, 5µg/ml） overnight at +4°C. The secondary antibody （green） was ab96879 Dylight 488 goat anti-mouse IgG （H+L） used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes （red） at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei （blue） at a concentration of 1.43µM.
  
  

References for Anti-Cdk7 antibody [MO1.1] （ab9516）