Anti-AMACR，α－甲基酰基辅酶A消旋酶抗体

• Product nameAnti-AMACR antibody [2A10F3]See all AMACR primary antibodies ...
• Description
  Mouse monoclonal [2A10F3] to AMACR
• Tested applicationsICC/IF, WB, ELISA, IHC-P, Flow Cyt more details
• Species reactivity
  Reacts with: Human
• Immunogen

  Purified truncated recombinant AMACR-His fusion protein expressed in E. Coli strain BL21 （DE3）

• Positive controlJurkat Cell lysate. Prostate adenocarcinoma.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot ａnd store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: Ascites
• PurityAscites
• ClonalityMonoclonal
• Clone number2A10F3
• IsotypeIgG2b
• Research Areas
  • Tags & Cell Markers
  • Cell Type Markers
  • Tumor Associated

  • Epigenetics ａnd Nuclear Signaling
  • Transcription
  • Cancer susceptibility
  • Proto-oncogenes

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids ａnd lipoproteins

  • Metabolism
  • Pathways ａnd Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways ａnd Processes
  • Metabolic signaling pathways
  • Lipid ａnd lipoprotein metabolism
  • Lipid metabolism

  • Metabolism
  • Pathways ａnd Processes
  • Redox metabolism
  • Oxidative stress

  • Metabolism
  • Types of disease
  • Cancer

Applications

Target

• FunctionRacemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA ａnd C27-bile acyl-CoAs to their （S）-stereoisomers.
• PathwayLipid metabolism; bile acid biosynthesis.
  Lipid metabolism; fatty acid metabolism.
• Involvement in diseaseDefects in AMACR are the cause of alpha-methylacyl-CoA racemase deficiency （AMACRD） [MIM:614307]. AMACRD results in elevated plasma concentrations of pristanic acid C27-bile-acid intermediates. It can be associated with polyneuropathy, retinitis pigmentosa, epilepsy.
  Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 （CBAS4） [MIM:214950]; also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency ａnd absence of cholic acid from bile.
• Sequence similaritiesBelongs to the CaiB/BaiF CoA-transferase family.
• Cellular localizationPeroxisome. Mitochondrion.
• Information by UniProt
• Database links
  • Entrez Gene: 23600 Human
  • Omim: 604489 Human
  • SwissProt: Q9UHK6 Human
  • Unigene: 508343 Human
• Alternative names
  • 2 arylpropionyl CoA epimerase antibody
  • 2 methylacyl CoA racemase antibody
  • 2-methylacyl-CoA racemase antibody

  see all

Anti-AMACR antibody [2A10F3] images

• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - AMACR antibody [2A10F3] （ab63340）
  ab6330 staining AMACR by immunohistochemistry on paraffin-embedded human normal prostate tissue （left, light staining） ａnd on prostate adenocarcinoma using DAB staining （right）
• 

  Immunocytochemistry/ Immunofluorescence - AMACR antibody [2A10F3] （ab63340）

  ab63340 at 1/1000 dilution staining AMACR in LnCap cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated goat anti-mouse {IgG （H+C）} was used as secondary. Actin filaments were stained red with DY-554 phalloidin. Nuclei were stained blue with DRAQ5 fluorescent DNA dye.

• 

  Western blot - AMACR antibody [2A10F3] （ab63340）
  All lanes : Anti-AMACR antibody [2A10F3] （ab63340） at 1/2000 dilution
  
  Lane 1 : Cell lysates prepared from Jurkat cells
  Lane 2 : Cell lysates prepared from LnCaP cells
  
  Lysates/proteins at 100 µg per lane.
  
  Secondary
  HRP-conjugated Goat polyclonal to mouse IgG1
  
  Predicted band size : 42 kDa
  
• 

  Flow Cytometry - Anti-AMACR antibody [2A10F3] （ab63340）
  Overlay histogram showing PC3 cells stained with ab63340 （red line）. The cells were fixed with 80% methanol （5 min） ａnd then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody （ab63340, 1µg/1x10⁶ cells） for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG （H+L） （ab96879） at 1/500 dilution for 30 min at 22ºC. Isotype control antibody （black line） was mouse IgG2b [PLPV219] （ab91366, 2µg/1x10⁶ cells） used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC3 cells fixed with 4% paraformaldehyde （10 min）/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  
  

References for Anti-AMACR antibody [2A10F3] （ab63340）