Anti-CDK5,周期素依赖性激酶5抗体

• Product nameAnti-Cdk5 antibodySee all Cdk5 primary antibodies ...
• Description
  Rabbit polyclonal to Cdk5
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Sheep, Chicken, Cow, Pig, Zebrafish
• Immunogen

  Synthetic peptide corresponding to a region within N terminal amino acids 1-50 （MQKYEKLEKI GEGTYGTVFK AKNRETHEIV ALKRVRLDDD DEGVPSSALR） of human Cdk5 （NP_004926）.

  Run BLAST with Run BLAST with
• Positive control721_B cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot ａnd store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration50 µg at 1 mg/ml
  
• PurityImmunogen affinity purified
• Purification notesPurified by peptide affinity chromatography method.
• ClonalityPolyclonal
• IsotypeIgG
• Research Areas
  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Cdks

  • Neuroscience
  • Neurology process
  • Neurogenesis

  • Cancer
  • Cell cycle
  • Cell division

  • Metabolism
  • Types of disease
  • Obesity

Applications

• Application notesELISA: Use at an assay dependent dilution.
  ELISA titre using peptide based assay, 1:62500
  WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.
  
  Not yet tested in other applications.
  Optimal dilutions/concentrations should be determined by the end user.

Target

• FunctionProbably involved in the control of the cell cycle. Interacts with D1 ａnd D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 ａnd NF-H ａnd NF-M. Also interacts with p35 which activates the kinase.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
  Contains 1 protein kinase domain.
• Cellular localizationCytoplasm. Cell projection > lamellipodium. Cell projection > growth cone. In axonal growth cone with extension to the peripheral lamellipodia.
• Information by UniProt
• Database links
  • Entrez Gene: 281066 Cow
  • Entrez Gene: 1020 Human
  • Entrez Gene: 12568 Mouse
  • Entrez Gene: 140908 Rat
  • Omim: 123831 Human
  • SwissProt: Q02399 Cow
  • SwissProt: Q00535 Human
  • SwissProt: P49615 Mouse

  see all
• Alternative names
  • Cdk 5 antibody
  • Cdk5 antibody
  • CDK5_HUMAN antibody

  see all

Anti-Cdk5 antibody images

• 

  Western blot - Cdk5 antibody （ab83022）
  Anti-Cdk5 antibody （ab83022） at 1 µg/ml + 721_B cell lysate at 10 µg
  
  Secondary
  HRP conjugated anti-Rabbit IgG at 1/50000 dilution
  
  Predicted band size : 33 kDa
  Observed band size : 33 kDa
  

References for Anti-Cdk5 antibody （ab83022）