Anti-CDK1,周期素依赖性激酶1抗体

• Product nameAnti-CDK1 [A17] antibodySee all CDK1 primary antibodies ...
• Description
  Mouse monoclonal [A17] to CDK1
• Conjugation notes-
• SpecificityThe antibody inhibits the activation of p34cdc2 kinase by cyclins.
• Tested applicationsICC/IF, Flow Cyt, WB, IP, ELISA, IHC-FoFr, IHC-P, RIA, IHC-Fr more details
• Species reactivity
  Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
• Immunogen

  Carboxyterminal fragment starting at aa 85 of Xenopus p34cdc2 expressed in E. coli

• EpitopeThe epitope is thought to be residues 220-227 of mouse cdc2, LGTPNNEV.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term （1-2 weeks）. Aliquot ａnd store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS
• Concentration100 µg at 1 mg/ml 100 µg at 1 mg/ml
  
• PurityImmunogen affinity purified
• ClonalityMonoclonal
• Clone numberA17
• MyelomaSp2
• IsotypeIgG2a
• Light chain typeunknown
• Research Areas
  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Cdks

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

  • Epigenetics ａnd Nuclear Signaling
  • Cell cycle
  • Kinases/Phosphatases
  • Cdks

Applications

Target

• FunctionPlays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase ａnd mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
• Tissue specificityIsoform 2 is found in breast cancer tissues.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
  Contains 1 protein kinase domain.
• Cellular localizationNucleus.
• Information by UniProt
• Database links
  • Entrez Gene: 396252 Chicken
  • Entrez Gene: 983 Human
  • Entrez Gene: 12534 Mouse
  • Entrez Gene: 54237 Rat
  • Entrez Gene: 380246 Xenopus laevis
  • Omim: 116940 Human
  • SwissProt: P13863 Chicken
  • SwissProt: P06493 Human

  see all
• FormCDK1 can be located to the Nucleus, cytoplasm ａnd Mithocondria. It＇s cytoplasmic during interphase ａnd reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
• Alternative names
  • Cdc 2 antibody
  • Cdc2 antibody
  • CDC28A antibody

  see all

Anti-CDK1 [A17] antibody images

• 

  Western blot - Cdc2 antibody [A17] （ab18）
  All lanes : Anti-CDK1 [A17] antibody （ab18）
  
  Lane 1 : Rapidly dividing Swiss 3T3 cells
  Lane 2 : Quiescent Swiss 3T3 cells
  
  
  Predicted band size : 34 kDa
  Observed band size : 34 kDa
  
• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Cdc2 antibody [A17] （ab18）
  Ab18 staining human skin. Staining is localized to the cytoplasm ａnd nucleus.
  Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system （Dako PT Link）, at room temperature. Sections were rehydrated ａnd antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes （containing casein 0.25% in PBS） then incubated with primary antibody for 20 minutes ａnd detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin ａnd coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration ａnd incubation time （overnight incubation）, ａnd amplification may be required.
• 

  Immunocytochemistry/ Immunofluorescence - Cdc2 antibody [A17] （ab18）

  See Abreview

• 

  Flow Cytometry-Anti-CDK1 antibody [A17]（ab18）
  Overlay histogram showing MCF7 cells stained with ab18 （red line）. The cells were fixed with 4% paraformaldehyde （10 min） ａnd then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody （ab18, 0.5µg/1x10⁶ cells） for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG （H+L） （ab96879） at 1/500 dilution for 30 min at 22ºC. Isotype control antibody （black line） was mouse IgG2a [ICIGG2A] （ab91361, 1µg/1x10⁶ cells） used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol （5 min）/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  
  
• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Anti-CDK1 antibody [A17] （ab18）Image from Zhang C et al., PLoS One. 2011;6（8）:e23849. Epub 2011 Aug 24. Fig 3.; doi:10.1371/journal.pone.0023849; August 24, 2011, PLoS ONE 6（8）: e23849.
  Immunohistochemical analysis of Human lung cancer tissue, staining CDK1 with ab18 at 1/100 dilution.

References for Anti-CDK1 [A17] antibody （ab18）