Anti-CD79A,免疫球蛋白结合蛋白-1抗体

• Product nameAnti-CD79a antibody [HM47/A9]See all CD79a primary antibodies ...
• Description
  Mouse monoclonal [HM47/A9] to CD79a
• SpecificityThis antibody labels precursor B-cell acute lymphoblastic leukemia samples, making it the most reliable B-cell marker for this disorder. In a study by Mason, et al on a total of 454 paraffin-embedded tissues, it reacted with 97% of B-cell neoplasms. We have data to indicate that this antibody may not cross react with Opossum. However, this has not been conclusively tested ａnd expression levels may vary in certain cell lines/tissues.
• Tested applicationsICC/IF, IHC-P, WB, Flow Cyt more details
• Species reactivity
  Reacts with: Mouse, Rat, Cow, Human, Pig, Monkey
  Predicted to work with: Rabbit, Horse, Chicken, Guinea pig
• Immunogen

  Synthetic peptide: GTYQDVGSLNIADVQ, corresponding to C terminal amino acids 208-222 of Human CD79a.

  Run BLAST with Run BLAST with
• Positive controlRaji cells. Lymph node. Tonsil.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot ａnd store at -20°C. Avoid freeze / thaw cycles.
• Storage buffer10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
• Concentration500 µl at 0.2 mg/ml
  
• PurityProtein G purified
• ClonalityMonoclonal
• Clone numberHM47/A9
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Immunology
  • Adaptive Immunity
  • B Cells
  • CD

  • Immunology
  • Immunoglobulins
  • Receptors

  • Cancer
  • Tumor immunology
  • CD markers

  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • B Lymphocytic Lineage

Applications

Target

• FunctionRequired in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex （BCR） which leads to internalization of the complex, trafficking to late endosomes ａnd antigen presentation. Also required for BCR surface expression ａnd for efficient differentiation of pro- ａnd pre-B-cells. Stimulates SYK autophosphorylation ａnd activation. Binds to BLNK, bringing BLNK into proximity with SYK ａnd allowing SYK to phosphorylate BLNK. Also interacts with ａnd increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.
• Tissue specificityB-cells.
• Involvement in diseaseDefects in CD79A are the cause of agammaglobulinemia type 3 （AGM3） [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies ａnd low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 ａnd the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein.
• Sequence similaritiesContains 1 Ig-like C2-type （immunoglobulin-like） domain.
  Contains 1 ITAM domain.
• Post-translational
  modificationsPhosphorylated on tyrosine, serine ａnd threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early ａnd essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine ａnd threonine residues may prevent subsequent tyrosine phosphorylation.
• Cellular localizationCell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
• Information by UniProt
• Database links
  • Entrez Gene: 281674 Cow
  • Entrez Gene: 100052219 Horse
  • Entrez Gene: 973 Human
  • Entrez Gene: 12518 Mouse
  • Entrez Gene: 100190992 Pig
  • Entrez Gene: 681236 Rat
  • Omim: 112205 Human
  • SwissProt: P40293 Cow

  see all
• Alternative names
  • B cell antigen receptor complex associated protein alpha chain antibody
  • B cell antigen receptor complex associated protein alpha chain precursor antibody
  • B Lymphocyte Specific MB1 Protein antibody

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Anti-CD79a antibody [HM47/A9] images

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  Flow Cytometry - Anti-CD79a antibody [HM47/A9] （ab3121）

  Human peripheral blood lymphocytes stained with ab3121 （red line）. Human whole blood was processed using a modified protocol based on Chow et al, 2005 （PMID: 16080188）. In brief, human whole blood was fixed in 4% formaldehyde （methanol-free） for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 （final concentration - 0.1%） for 15 min at 37°C. For experimentation, cells were treated with 50% methanol （-20°C） for 15 min at 4°C. Cells were then incubated with the antibody （ab3121, 0.01μg/1x10⁶ cells） for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG （H&L） （ab150113） at 1/2000 dilution for 30 min at 4°C. Isotype control antibody （black line） was mouse IgG1 [ICIGG1] （ab91353, 1μg/1x10⁶ cells） used under the same conditions. Unlabelled sample （blue line） was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser （488nm） ａnd 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

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  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - CD79a antibody [HM47/A9] （ab3121）This image is courtesy of an anonymous Abreview
  ab3121 at 1/200 staining rat lymphoid tissues sections （Spleen, Lymph Node, Payers Patch） by IHC-P. The tissue was formaldehyde fixed ａnd blocked before incubation with the antibody for 1 hour. A rabbit anti-mouse antibody was used as the secondary.

  See Abreview

• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - CD79a antibody [HM47/A9] （ab3121）This image is courtesy of an Abreview submitted by Carl Hobbs

  ab3121 staining human tonsil sections by IHC-P. The tissue was formaldehyde fixed ａnd blocked before incubation for 10 minutes with 1%BSA, followed by incubations with ab3121 at 1/1000for 1h . A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary.

  See Abreview

• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - CD79a antibody [HM47/A9] （ab3121）This image is courtesy of an Abreview submitted by Carl Hobbs

  ab3121 staining monkey spleen sections by IHC-P. The tissue was formaldehyde fixed ａnd blocked before incubation for 10 minutes with 1%BSA, followed by incubations with ab3121 at 1/1000for 1h. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary.

  See Abreview

References for Anti-CD79a antibody [HM47/A9] （ab3121）