Anti-CD2AP,白细胞分化抗原CD2AP抗体

• Product nameAnti-CD2AP antibodySee all CD2AP primary antibodies ...
• Description
  Rabbit polyclonal to CD2AP
• Tested applicationsWB, ICC/IF, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human CD2AP.

  Read Abcam＇s proprietary immunogen policy

  （Peptide available as ab32740.）

• Positive controlHeLa （Human epithelial carcinoma cell line） Whole Cell Lysate Jurkat （Human T cell lymphoblast-like cell line） Whole Cell Lysate A431 （Human epithelial carcinoma cell line） Whole Cell Lysate MEF1 （Mouse embryonic fibroblast cell line） Whole Cell Lysate Spinal Cord （Mouse） Tissue Lysate Brain （Rat） Tissue Lysate

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term （1-2 weeks）. Aliquot ａnd store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• ConcentrationBatch dependent within range: 100 µg at 0.5 - 1 mg/ml
  

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• PurityImmunogen affinity purified
• ClonalityPolyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin Binding Proteins

  • Signal Transduction
  • Protein Trafficking
  • Organelle Proteins

Applications

Target

• FunctionSeems to act as an adapter protein between membrane proteins ａnd the actin cytoskeleton. May play a role in receptor clustering ａnd cytoskeletal polarity in the junction between T-cell ａnd antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis.
• Tissue specificityWidely expressed in fetal ａnd adult tissues.
• Involvement in diseaseDefects in CD2AP are the cause of susceptibility to focal segmental glomerulosclerosis type 3 （FSGS3） [MIM:607832]. A renal pathology defined by the presence of segmental sclerosis in glomeruli ａnd resulting in proteinuria, reduced glomerular filtration rate ａnd edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
• Sequence similaritiesContains 3 SH3 domains.
• DomainThe Pro-rich domain may mediate binding to SH3 domains.
  Potential homodimerization is mediated by the coiled coil domain.
• Post-translational
  modificationsPhosphorylated on tyrosine residues; probably by c-Abl, Fyn ａnd c-Src.
• Cellular localizationCytoplasm > cytoskeleton. Cell projection > ruffle. Colocalizes with F-actin ａnd BCAR1/p130Cas in membrane ruffles. Located at podocyte slit diaphragm between podocyte foot processes （By similarity）. During late anaphase ａnd telophase, concentrates in the vicinity of the midzone microtubules ａnd in the midbody in late telophase.
• Information by UniProt
• Database links
  • Entrez Gene: 23607 Human
  • Entrez Gene: 12488 Mouse
  • Entrez Gene: 316258 Rat
  • Omim: 604241 Human
  • SwissProt: Q9Y5K6 Human
  • SwissProt: Q9JLQ0 Mouse
  • Unigene: 485518 Human
  • Unigene: 218637 Mouse

  see all
• Alternative names
  • Adapter protein CMS antibody
  • AL024079 antibody
  • C78928 antibody

  see all

Anti-CD2AP antibody images

• 

  Western blot - CD2AP antibody （ab32741）
  All lanes : Anti-CD2AP antibody （ab32741） at 1 µg/ml
  
  Lane 1 : HeLa （Human epithelial carcinoma cell line） Whole Cell Lysate （ab27252）
  Lane 2 : Jurkat （Human T cell lymphoblast-like cell line） Whole Cell Lysate （ab7899）
  Lane 3 : A431 （Human epithelial carcinoma cell line） Whole Cell Lysate （ab7909）
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  IR Dye 680 Conjugated Goat Anti-Rabbit IgG （H&L） at 1/15000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 71 kDa
  Observed band size : 74 kDa （why is the actual band size different from the predicted?）
  
• 

  Western blot - CD2AP antibody （ab32741）
  All lanes : Anti-CD2AP antibody （ab32741） at 1 µg/ml
  
  Lane 1 : MEF1 （Mouse embryonic fibroblast cell line） Whole Cell Lysate
  Lane 2 : Spinal Cord （Mouse） Tissue Lysate
  Lane 3 : Brain （Rat） Tissue Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  IRDye 680 Conjugated Goat Anti-Rabbit IgG （H+L） at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 71 kDa
  Observed band size : 74 kDa （why is the actual band size different from the predicted?）
  
• 

  Immunocytochemistry/ Immunofluorescence - CD2AP antibody （ab32741）
  ICC/IF image of ab32741 stained mouse embryonic fibroblast cells. The cells were methanol fixed （5 min）, permabilised in TBS-T （20 min） and incubated with the antibody （ab32741, 1µg/ml） for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence ａnd block non-specific protein-protein interactions. The secondary antibody （green） was Alexa Fluor® 488 goat anti-rabbit IgG （H+L） used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes （red）. DAPI was used to stain the cell nuclei （blue）.
• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Anti-CD2AP antibody （ab32741）

References for Anti-CD2AP antibody （ab32741）