Anti-Calpain,钙蛋白酶抗体

• Product nameAnti-Calpain 1 antibodySee all Calpain 1 primary antibodies ...
• Description
  Rabbit polyclonal to Calpain 1
• Specificityab28258 recognizes Domain I of the large subunit of Calpain 1. The antibody does not recognize amino-processed Calpain 1, ａnd can be used to discriminate between latent ａnd amino-processed forms. Also ab28258 does not recognize the other calpain family members （M calpain, calpain 94, ncl 2, ncl 3, etc.）.
• Tested applicationsWB, ICC, Flow Cyt, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
• Immunogen

  Synthetic peptide based on Domain I of the large subunit of human calpain 1. （Peptide available as ab41299.）

• Epitopeab28258 reacts with the propeptide domain, （Domain I） of Calpain 1, immediately aminoterminal from the activation cleavage site.
• Positive controlCalpain 1

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term （1-2 weeks）. Aliquot ａnd store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 50% Glycerol, PBS
• Concentration100 µg at 1 mg/ml
  
• PurityImmunogen affinity purified
• Purification notesThe antibody has been peptide-affinity purified.
• ClonalityPolyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Signaling Pathway
  • Calcium Signaling
  • Calpain

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteolytic enzymes
  • Cysteine protease
  • Calpains

Applications

Target

• FunctionCalcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling ａnd signal transduction.
• Tissue specificityUbiquitous.
• Sequence similaritiesBelongs to the peptidase C2 family.
  Contains 1 calpain catalytic domain.
  Contains 4 EF-hand domains.
• Cellular localizationCytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca（2+） binding.
• Information by UniProt
• Database links
  • Entrez Gene: 823 Human
  • Entrez Gene: 12333 Mouse
  • Entrez Gene: 29153 Rat
  • Omim: 114220 Human
  • SwissProt: P07384 Human
  • SwissProt: O35350 Mouse
  • SwissProt: P97571 Rat
  • Unigene: 502842 Human

  see all
• Alternative names
  • Ca2 activated neutral protease antibody
  • Calcium activated neutral proteinase 1 antibody
  • Calcium activated neutral proteinase antibody

  see all

Anti-Calpain 1 antibody images

• 

  Western blot - Calpain 1 antibody - Domain I （ab28258）
  Anti-Calpain 1 antibody （ab28258） at 1/1000 dilution + Calpain 1 at 10 µg
  
  Predicted band size : 82 kDa
  Observed band size : 80 kDa （why is the actual band size different from the predicted?）
  
• 

  Immunocytochemistry/ Immunofluorescence - Calpain 1 antibody - Domain I （ab28258）This image is courtesy of an anonymous Abreview

  ab28258 staining human HUVEC cells by ICC/IF. Cells were PFA fixed ａnd permeablized in 0.5% Triton X-100 prior to blocking in 1% serum for 16 hours at 4°C. The primary antibody was diluted 1/100 ａnd incubated with the sample for 2 hours at 25°C. A FITC conjugated goat anti-rabbit antibody, diluted 1/100, was used as the secondary.

  See Abreview

• 

  Flow Cytometry - Calpain 1 antibody - Domain I （ab28258）This image is courtesy of a Mahesh Shivananjappa.
  ab28258 detecting Calpain 1 - Domain I in Human platelets by Flow Cytometry. Platelets were isolated by spinning Platelet rich plasma on Histopaque. Cells were fixed in PFA ａnd permeabilized in 0.1% Triton-X100 in 2% BSA for 30 minutes. The primary antibody was diluted 1/250 in 2% BSA in PBS ａnd incubated with the sample for 18 hours at 4°C. An Alexa Fluor® 488-conjugated Chicken anti-Rabbit IgG （H+L） antibody, diluted 1/500 was used as the secondary antibody.

  Abbreviations in the figure:
  P: Permeabilized;
  US Unstained, Red Peak;
  IgG Rb: IgG Rabbit （Isotype Control）, Blue Peak;
  Calpain: Green Peak.

  See Abreview

Protocols

• Recommended WB protocol

References for Anti-Calpain 1 antibody （ab28258）