Anti-CAD,Caspase 激活的脱氧核糖核酸酶抗体

• Product nameAnti-CAD antibody [EP710Y]See all CAD primary antibodies ...
• Description
  Rabbit monoclonal [EP710Y] to CAD
• Specificityab40800 is specific to the N-terminus of CAD.
• Tested applicationsICC/IF, WB, Flow Cyt, IP more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
• Immunogen

  Synthetic peptide corresponding to residues in the N-terminus of human CAD.

• Positive control293 or HeLa cells.
• General notesProduced under U.S. Patent No. 5,675,063. Stable from 12 months of receipt.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot ａnd store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• ClonalityMonoclonal
• Clone numberEP710Y
• IsotypeIgG
• Research Areas
  • Cell Biology
  • Apoptosis
  • Nucleus
  • DNAses

  • Epigenetics ａnd Nuclear Signaling
  • DNA / RNA
  • DNA / Nucleotides

  • Epigenetics ａnd Nuclear Signaling
  • Cell cycle
  • Apoptosis
  • Nuclear

  • Metabolism
  • Pathways ａnd Processes
  • Metabolic signaling pathways
  • Nucleotide metabolism

  • Metabolism
  • Pathways ａnd Processes
  • Metabolism processes
  • Apoptosis

Applications

Target

• RelevanceCarbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase （CAD） is a multifunctional protein that initiates ａnd regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK ａnd EGF phosphorylate CAD at threonine 456, MAPK ａnd c-myc have been found to induce over-expression of CAD.
• Cellular localizationCytoplasmic ａnd Nuclear
• Database links
  • Entrez Gene: 790 Human
  • Entrez Gene: 69719 Mouse
  • Entrez Gene: 24240 Rat
  • Omim: 114010 Human
  • SwissProt: P27708 Human
  • SwissProt: B2RQC6 Mouse
  • Unigene: 377010 Human
  • Unigene: 19416 Rat
• Alternative names
  • Aspartate carbamoyltransferase antibody
  • Aspartate transcarbamylase antibody
  • CAD protein antibody

  see all

Anti-CAD antibody [EP710Y] images

• 

  Western blot - CAD protein antibody [EP710Y] （ab40800）
  Anti-CAD antibody [EP710Y] （ab40800） at 1/1000 dilution + HEK293 Human embryonic kidney cell line Whole Cell Lysate （ab7902） at 10 µg
  
  Predicted band size : 243 kDa
  Observed band size : 250 kDa （why is the actual band size different from the predicted?）
  
• 

  Immunofluorescence - CAD protein antibody [EP710Y] （ab40800）ab40800 （1/100）, staining human CAD （N-term） in HeLA cells by Immunofluorescence.
• 

  Flow Cytometry - CAD antibody [EP710Y] （ab40800）

  Overlay histogram showing HeLa cells stained with ab40800 （red line）. The cells were fixed with 80% methanol （5 min） ａnd then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody （ab40800, 1/100 dilution） for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG （H+L） （ab96899） at 1/500 dilution for 30 min at 22°C. Isotype control antibody （black line） was rabbit IgG （monoclonal） （1μg/1x10⁶ cells） used under the same conditions. Acquisition of >5,000 events was performed.
  

References for Anti-CAD antibody [EP710Y] （ab40800）