anti-Bak,Bcl-2同源拮抗剂Bak抗体

• Product nameAnti-Bak antibodySee all Bak primary antibodies ...
• Description
  Rabbit polyclonal to Bak
• Tested applicationsWB, IP, ICC/IF more details
• Species reactivity
  Reacts with: Hamster, Human, Pig, Monkey
• Immunogen

  Synthetic peptide derived from the sequence of human Bak, conjugated to KLH.

• Positive controlA431 or HeLa cells. Cervical cancer tissue.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot ａnd store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: Borate buffered saline, pH 8.2
• Concentration100 µg at 1 mg/ml
  
• PurityImmunogen affinity purified
• ClonalityPolyclonal
• IsotypeIgG
• Research Areas
  • Cell Biology
  • Apoptosis
  • Intracellular
  • Bcl2 Family

Applications

Target

• FunctionIn the presence of an appropriate stimulus, accelerates programmed cell death by binding to, ａnd antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.
• Tissue specificityExpressed in a wide variety of tissues, with highest levels in the heart ａnd skeletal muscle.
• Sequence similaritiesBelongs to the Bcl-2 family.
• DomainIntact BH3 motif is required by BIK, BID, BAK, BAD ａnd BAX for their pro-apoptotic activity ａnd for their interaction with anti-apoptotic members of the Bcl-2 family.
• Cellular localizationMitochondrion membrane.
• Information by UniProt
• Database links
  • Entrez Gene: 578 Human
  • Entrez Gene: 397543 Pig
  • Omim: 600516 Human
  • SwissProt: Q16611 Human
  • SwissProt: Q6I9T6 Human
  • Unigene: 485139 Human
• Alternative names
  • Apoptosis Regulator Bak antibody
  • BAK antibody
  • BAK like antibody

  see all

Anti-Bak antibody images

• 

  Western blot - Bak antibody （ab69404）
  Anti-Bak antibody （ab69404） at 1/1000 dilution + A431 cell lysate
  
  Predicted band size : 23 kDa
  Observed band size : 28 kDa （why is the actual band size different from the predicted?）
  
• 

  Immunocytochemistry/ Immunofluorescence - Bak antibody （ab69404）
  Immunofluorescent staining of HeLa cells using ab69404. nuclei were counter-stained using DRAQ5.
• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Bak antibody （ab69404）
  Immunohistochemical analysis of human cervical cancer tissue using 1/50 ab69404.

References for Anti-Bak antibody （ab69404）