Anti-Bad,相关死亡促进因子Bad抗体

• Product nameAnti-Bad antibodySee all Bad primary antibodies ...
• Description
  Rabbit polyclonal to Bad
• SpecificityThis antibody specifically binds the Bad protein, but does not recognize Bad/Bcl2 heterodimers in Immunoprecipitation assays.
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Hamster, Cow, Dog, Pig, Fruit fly （Drosophila melanogaster）, Monkey
• Immunogen

  Synthetic peptide derived from the sequence of Mouse Bad, conjugated to KLH.

• Positive controlWB: Mouse Raw 264 cell lysate, Rat brain tissue lysate IHC-P: Alzheimer diseased brain section IF/ICC: RAW246.7 cell line

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot ａnd store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 50% Glycerol, PBS, pH 7.2
• Concentration100 µg at 1 mg/ml
  
• PurityProtein A purified
• ClonalityPolyclonal
• IsotypeIgG
• Research Areas
  • Cell Biology
  • Apoptosis
  • Intracellular
  • Bcl2 Family

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Bcl 2 family

  • Metabolism
  • Pathways ａnd Processes
  • Metabolism processes
  • Apoptosis

Applications

Target

• FunctionPromotes cell death. Successfully competes for the binding to Bcl-X（L）, Bcl-2 ａnd Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X（L）, but not that of Bcl-2 （By similarity）. Appears to act as a link between growth factor receptor signaling ａnd the apoptotic pathways.
• Tissue specificityExpressed in a wide variety of tissues.
• Sequence similaritiesBelongs to the Bcl-2 family.
• DomainIntact BH3 motif is required by BIK, BID, BAK, BAD ａnd BAX for their pro-apoptotic activity ａnd for their interaction with anti-apoptotic members of the Bcl-2 family.
• Post-translational
  modificationsPhosphorylated on one or more of Ser⁻⁷5, Ser-99, Ser-118 ａnd Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser⁻⁷5 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X（L） ａnd the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A （CAPK） phosphorylation. Ser⁻⁷5 is phosphorylated by AKT/PKB, protein kinase A ａnd PIM2.
• Cellular localizationMitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
• Information by UniProt
• Database links
  • Entrez Gene: 12015 Mouse
  • Entrez Gene: 64639 Rat
  • SwissProt: Q61337 Mouse
  • SwissProt: O35147 Rat
  • Unigene: 4387 Mouse
  • Unigene: 36696 Rat
• Alternative names
  • BAD antibody
  • BAD_HUMAN antibody
  • BBC 2 antibody

  see all

Anti-Bad antibody images

• 

  Western blot - Bad antibody （ab90435）
  Anti-Bad antibody （ab90435） at 1 µg/ml + Mouse Raw 264 cell lysate
  developed using the ECL technique
  
  Predicted band size : 18 kDa
  
• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Bad antibody （ab90435）
  ab90435, at a 1/50 dilution, staining BAD in the neurons of paraffin embedded Alzheimer diseased brain tissue （right） by Immunohistochemistry. Control brain tissue （left）.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Bad antibody （ab90435）
  ICC/IF image of ab90435 stained RAW246.7 cells. The cells were 4% paraformaldehyde fixed （10 min） ａnd then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells ａnd block non-specific protein-protein interactions. The cells were then incubated with the antibody （ab90435, 5µg/ml） overnight at +4°C. The secondary antibody （green） was ab96899, DyLight® 488 goat anti-rabbit IgG （H+L） used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes （red） at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei （blue） at a concentration of 1.43µM.

References for Anti-Bad antibody （ab90435）