Anti-BACE,β分泌酶抗体

• Product nameAnti-BACE1 antibodySee all BACE1 primary antibodies ...
• Description
  Rabbit polyclonal to BACE1
• SpecificityMultiple isoforms of this protein are known to exist; this antibody will detect all isoforms. The glycosylated isoform may also be observed at 70kDa in SDS-PAGE.
• Tested applicationsICC, WB, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
• Immunogen

  Synthetic peptide, corresponding to amino acids 485-501 of Human BACE. The peptide sequence differs fromthose of mouse ａnd rat BACE by one amino acid （1）.

• Positive controlHuman brain tissue lysate, 3T3 lysate.
• General notes

  Beta-site APP Cleaving Enzyme.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot ａnd store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS with 0.02% sodium azide
• Concentration100 µg at 1 mg/ml
  
• PurityIgG fraction
• Primary antibody notesBeta-site APP Cleaving Enzyme.
• ClonalityPolyclonal
• IsotypeIgG
• Research Areas
  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer＇s disease
  • Proteases

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteolytic enzymes
  • Aspartic protease
  • BACEs

Applications

Target

• FunctionResponsible for the proteolytic processing of the amyloid precursor protein （APP）. Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 ａnd 672 of APP, leads to the generation ａnd extracellular release of beta-cleaved soluble APP, ａnd a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
• Tissue specificityExpressed at high levels in the brain ａnd pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus ａnd medulla oblongata.
• Sequence similaritiesBelongs to the peptidase A1 family.
• DomainThe transmembrane domain is necessary for its activity. It determines its late Golgi localization ａnd access to its substrate, APP.
• Post-translational
  modificationsGlycosylated.
• Cellular localizationMembrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network （TGN） ａnd minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes ａnd on the cell surface.
• Information by UniProt
• Database links
  • Entrez Gene: 23621 Human
  • Entrez Gene: 23821 Mouse
  • Entrez Gene: 29392 Rat
  • Omim: 604252 Human
  • SwissProt: P56817 Human
  • SwissProt: P56818 Mouse
  • SwissProt: P56819 Rat
  • Unigene: 504003 Human

  see all
• Alternative names
  • APP beta secretase antibody
  • Asp 2 antibody
  • ASP2 antibody

  see all

Anti-BACE1 antibody images

• 

  Western blot - BACE1 antibody （ab2077）
  All lanes : Anti-BACE1 antibody （ab2077） at 1 µg/ml
  
  Lane 1 : Human brain tissue lysate with absence of blocking peptide
  Lane 2 : Human brain tissue lysate with BACE1 peptide （ab7883）
  Lane 3 : Mouse 3T3 cell lysate
  
  
  Observed band size : 70 kDa （why is the actual band size different from the predicted?）
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody （ab2077）Image courtesy of an anonymous Abreview.
  ab2077 staining BACE1 in human glioblastoma cell line D54MG by Immunocytochemistry/ Immunofluorescence.
  
  Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab2077 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-BACE1 antibody （ab2077）Image courtesy of an anonymous Abreview.
  ab2077 staining BACE1 in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.
  
  Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab2077 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-rabbit polyclonal, used at a 1/400 dilution. Nuclei are counterstained with DAPI.

  See Abreview

References for Anti-BACE1 antibody （ab2077）