Anti-ATF3,活化转录因子3抗体

• Product nameAnti-ATF3 antibody [44C3a]See all ATF3 primary antibodies ...
• Description
  Mouse monoclonal [44C3a] to ATF3
• Tested applicationsWB, Dot Blot, ICC/IF, IHC-Fr, IHC-P more details
• Species reactivity
  Reacts with: Human
• Immunogen

  Recombinant fragment of Human ATF3

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term （1-2 weeks）. Aliquot ａnd store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 1% BSA, PBS （8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate）, pH 7.4
• Concentration100 µg at 0.1 mg/ml
  
• PurityProtein G purified
• Purification notesab58668 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted （approximately 95%） fetal bovine serum ａnd filtered through a 0.22µm membrane.
• ClonalityMonoclonal
• Clone number44C3a
• IsotypeIgG1
• Research Areas
  • Epigenetics ａnd Nuclear Signaling
  • Transcription
  • Other factors

  • Cancer
  • Tumor biomarkers
  • Other

Applications

Target

• FunctionThis protein binds the cAMP response element （CRE） （consensus: 5＇-GTGACGT[AC][AG]-3＇）, a sequence present in many viral ａnd cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
• Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
  Contains 1 bZIP domain.
• Cellular localizationNucleus.
• Information by UniProt
• Database links
  • Entrez Gene: 467 Human
  • Omim: 603148 Human
  • SwissProt: P18847 Human
  • Unigene: 460 Human
• Alternative names
  • Activating transcription factor 3 antibody
  • ATF3 antibody
  • ATF3_HUMAN antibody

  see all

Anti-ATF3 antibody [44C3a] images

• 

  Western blot - ATF3 antibody [44C3a] （ab58668）
  Anti-ATF3 antibody [44C3a] （ab58668） + immunising recombinant protein
  
  Predicted band size : 21 kDa
  Observed band size : 35 kDa （why is the actual band size different from the predicted?）
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATF3 antibody [44C3a] （ab58668）

  ab58668 staining ATF3 in A549 cells treated with ionomycin Ca²⁺ salt （ab120116）, by ICC/IF. Increase in ATF3 expression correlates with increased concentration of ionomycin Ca²⁺ salt, as described in literature.
  The cells were incubated at 37°C for 2h in media containing different concentrations of ab120116（ionomycin Ca²⁺ salt） in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature ａnd blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA ａnd 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 （10 µg/ml） was performed overnight at 4°C in PBS containing 1% BSA ａnd 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody （ab96879） at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI ａnd are shown in blue.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATF3 antibody [44C3a] （ab58668）
  ab58668 staining ATF3 in A549 cells treated with ionomycin （free acid） （ab120370）, by ICC/IF. Increase in ATF3 expression correlates with increased concentration of ionomycin （free acid）, as described in literature.
  The cells were incubated at 37°C for 2h in media containing different concentrations of ab120370（Ionomycin （free acid）） in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature ａnd blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA ａnd 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 （10 µg/ml） was performed overnight at 4°C in PBS containing 1% BSA ａnd 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody （ab96879） at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI ａnd are shown in blue.
  
• 

  Immunocytochemistry/ Immunofluorescence-Anti-ATF3 antibody [44C3a]（ab58668）
  ab58668 staining ATF3 in serum starved A549 cells treated with thapsigargin （ab120286）, by ICC/IF. Increase of ATF3 correlates with increased concentration of thapsigargin, as described in literature.
  The cells were incubated at 37°C for 1h in media containing different concentrations of ab120286（thapsigargin） in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature ａnd blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA ａnd 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 （10 µg/ml） was performed overnight at 4°C in PBS containing 1% BSA ａnd 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody （ab96879） at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI ａnd are shown in blue.
  

References for Anti-ATF3 antibody [44C3a] （ab58668）