Anti-ATF2,活化复制因子2抗体

• Product nameAnti-ATF2 antibodySee all ATF2 primary antibodies ...
• Description
  Rabbit polyclonal to ATF2
• SpecificityATF2 antibody detects endogenous levels of ATF2 protein around Threonine 71 or 53.
• Tested applicationsWB, IHC-P, ELISA, IP more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
• Immunogen

  The antiserum was produced against synthesized non-phosphopeptide derived from human ATF2 around the phosphorylation site of Threonine 71 or 53.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
• Concentration100 µg at 1 mg/ml
  
• ClonalityPolyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics ａnd Nuclear Signaling
  • Transcription
  • Domain Families
  • HLH / Leucine Zipper
  • Leucine Zipper

  • Immunology
  • Innate Immunity
  • TLR Signaling

Applications

Target

• FunctionTranscriptional activator, probably constitutive, which binds to the cAMP-responsive element （CRE） （consensus: 5＇-GTGACGT[AC][AG]-3＇）, a sequence present in many viral ａnd cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements （TRES） as part of an ATF2/JUN complex.
• Tissue specificityAbundant expression seen in the brain.
• Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
  Contains 1 bZIP domain.
  Contains 1 C2H2-type zinc finger.
• Post-translational
  modificationsPhosphorylation of Thr-69 ａnd Thr⁻⁷1 by MAPK14 causes increased transcriptional activity. Also phosphorylated ａnd activated by JNK.
• Cellular localizationNucleus.
• Information by UniProt
• Database links
  • Entrez Gene: 1386 Human
  • Entrez Gene: 100047997 Mouse
  • Entrez Gene: 11909 Mouse
  • Entrez Gene: 81647 Rat
  • Omim: 123811 Human
  • SwissProt: P15336 Human
  • SwissProt: P16951 Mouse
  • SwissProt: Q00969 Rat

  see all
• Alternative names
  • Activating Transcription Factor 2 antibody
  • Activating transcription factor 2 splice variant ATF2 var2 antibody
  • ATF 2 antibody

  see all

Anti-ATF2 antibody images

• 

  Western blot - ATF2 antibody （ab47476）
  All lanes : Anti-ATF2 antibody （ab47476） at 1/500 dilution
  
  Lane 1 : extracts from HeLa cells.
  Lane 2 : extracts from HeLa cells preincubated with immunizing peptide
  
  
  Predicted band size : 52 kDa
  Observed band size : 52 kDa
  
• 

  Immunohistochemistry （Paraffin-embedded sections） - ATF2 antibody （ab47476）
  Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using ATF2 antibody; Right: The same antibody preincubated with synthesized peptide.

References for Anti-ATF2 antibody （ab47476）