Anti-Aromatase,芳香化酶抗体

• Product nameAnti-Aromatase antibodySee all Aromatase primary antibodies ...
• Description
  Rabbit polyclonal to Aromatase
• Specificityab18995 recognises aromatase.
• Tested applicationsICC/IF, IHC-Fr, IHC-P, WB more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  Predicted to work with: Chicken, Cow, Pig
• Immunogen

  Synthetic peptide surrounding amino acid 385 of human Aromatase （Peptide available as ab51924.）

• Positive controlBrain, placenta ａnd gonads.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot ａnd store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Thimerosal （merthiolate）
  Constituents: 30% Glycerol, 0.5% BSA, PBS
• Concentration50 µg at 0.2 mg/ml
  
• PurityImmunogen affinity purified
• ClonalityPolyclonal
• IsotypeIgG
• Research Areas
  • Cell Biology
  • Cell Cycle
  • Cell differentiation

  • Neuroscience
  • Endocrine system
  • Gonadotrophic axis

  • Epigenetics ａnd Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Estrogen

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids ａnd lipoproteins

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Hormone biosynthesis

  • Metabolism
  • Pathways ａnd Processes
  • Metabolic signaling pathways
  • Lipid ａnd lipoprotein metabolism
  • Lipid metabolism

  • Metabolism
  • Pathways ａnd Processes
  • Endocrine metabolism
  • Hormone biosynthesis

  • Metabolism
  • Types of disease
  • Cancer

Applications

Target

• FunctionCatalyzes the formation of aromatic C18 estrogens from C19 androgens.
• Tissue specificityBrain, placenta ａnd gonads.
• Involvement in diseaseDefects in CYP19A1 are a cause of aromatase excess syndrome （AEXS） [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
  Defects in CYP19A1 are the cause of aromatase deficiency （AROD） [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia ａnd primary amenorrhoea with multicystic ovaries.
• Sequence similaritiesBelongs to the cytochrome P450 family.
• Cellular localizationMembrane.
• Information by UniProt
• Database links
  • Entrez Gene: 414854 Chicken
  • Entrez Gene: 1588 Human
  • Entrez Gene: 13075 Mouse
  • Entrez Gene: 25147 Rat
  • Omim: 107910 Human
  • SwissProt: P19098 Chicken
  • SwissProt: P11511 Human
  • SwissProt: P28649 Mouse

  see all
• Alternative names
  • ARO 1 antibody
  • ARO antibody
  • ARO1 antibody

  see all

Anti-Aromatase antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Aromatase antibody （ab18995）
  ICC/IF image of ab18995 stained HepG2 cells. The cells were 4% formaldehyde fixed （10 min） ａnd then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells ａnd block non-specific protein-protein interactions. The cells were then incubated with the antibody （ab18995, 1µg/ml） overnight at +4°C. The secondary antibody （green） was Alexa Fluor® 488 goat anti-rabbit IgG （H+L） used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes （red） at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei （blue） at a concentration of 1.43µM.
  
  
• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Aromatase antibody （ab18995）
  Ab18995 staining Human normal placenta. Staining is localised to cell membrane.
  Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated ａnd antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes （containing casein 0.25% in PBS） then incubated with primary antibody for 20 min ａnd detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin ａnd coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration ａnd incubation time （overnight incubation）, ａnd amplification may be required.
• 

  Western blot - Aromatase antibody （ab18995）
  Anti-Aromatase antibody （ab18995） at 1 µg/ml + Brain （Human） Membrane Lysate - adult normal tissue （ab29456） at 10 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L （HRP）, pre-adsorbed （ab97080） at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 58 kDa
  Observed band size : 58 kDa
  Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 1 minute

Protocols

• Recommended WB protocol with ab18995 ａnd ab22166

References for Anti-Aromatase antibody （ab18995）