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Anti-Ang-Ⅱ,血管紧张素II抗体

Anti-Ang-Ⅱ,血管紧张素II抗体

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价格:¥电议

更新日期:2026/8/7 19:44:07

生 产 地:美洲

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简单介绍:Anti-Ang-Ⅱ,血管紧张素II抗体
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相关标签:Anti-Ang-Ⅱ,血管紧张素II抗体,生物抗体,免疫抗体,进口抗体

详细内容

  • Product nameAnti-Angiotensin II Type 2 Receptor antibodySee all Angiotensin II Type 2 Receptor primary antibodies ...
  • Description
    Rabbit polyclonal to Angiotensin II Type 2 Receptor
  • Tested applicationsICC/IF, WB, Electron Microscopy, IHC-Fr, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit
  • Immunogen

    Synthetic peptide: CRKSSSLREMETFVS conjugated to KLH, corresponding to amino acids 349-363 of Rat Angiotensin II Type 2 Receptor.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration50 µg at 1.6 mg/ml
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research Areas
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • PLC
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cardiovascular
    • Atherosclerosis
    • Apoptosis
    • Cardiovascular
    • Blood
    • Blood Pressure regulation

Applications

Our Abpromise guarantee covers the use of ab19134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: Use a concentration of 1 µg/ml.
WB WB: 1/800. Detects a band of approximately 44 kDa. See Abreview. Control peptide isab91522.
Electron Microscopy EM: Use at an assay dependent dilution. PubMed: 19539732
IHC-Fr IHC-Fr: Use at an assay dependent dilution.
IHC-P IHC-P: 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionReceptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation.
  • Tissue specificityIn adult, highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. Very highly expressed in fetal kidney and intestine.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
    • Entrez Gene: 186 Human
    • Entrez Gene: 11609 Mouse
    • Entrez Gene: 24182 Rat
    • Omim: 300034 Human
    • SwissProt: P50052 Human
    • SwissProt: P35374 Mouse
    • SwissProt: Q3US12 Mouse
    • SwissProt: P35351 Rat
    see all
  • Alternative names
    • AGTR 2 antibody
    • AGTR2 antibody
    • AGTR2_HUMAN antibody
    see all

Anti-Angiotensin II Type 2 Receptor antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Angiotensin II Type 2 Receptor antibody (ab19134)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Angiotensin II Type 2 Receptor antibody (ab19134)This image is courtesy of an Abreview submitted by Miss Laura Palmer
    ab19134 at 1/2000 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum prior to incubation with the antibody for 18 hours. An HRP conjugated horse polyclonal antibody was used as the secondary.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Angiotensin II Type 2 Receptor antibody (ab19134)
    Immunocytochemistry/ Immunofluorescence - Angiotensin II Type 2 Receptor antibody (ab19134)
    ICC/IF image of ab19134 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Angiotensin II Type 2 Receptor antibody (ab19134)

Anti-Ang-Ⅱ,血管紧张素II抗体,生物抗体,免疫抗体,进口抗体

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