Anti-Agrin，聚集蛋白抗体

• Product nameAnti-Agrin antibodySee all Agrin primary antibodies ...
• Description
  Rabbit polyclonal to Agrin
• Tested applicationsIHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
• Immunogen

  Synthetic peptide from the N terminal residues of Human Agrin protein （NM_198576）.

• Positive controlHuman skeletal muscle.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot ａnd store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, 0.1M Tris glycine, pH 7.2
• Concentration100 µg at 0.5 mg/ml
  
• PurityImmunogen affinity purified
• ClonalityPolyclonal
• IsotypeIgG
• Research Areas
  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Growth Cone

  • Neuroscience
  • Cell Adhesion Proteins
  • ECM Proteins

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

Applications

• Application notesIHC-P: 1/100 - 1/500.
  Peptide ELISA titer: 1/10000 - 1/40000.
  
  
  Not yet tested in other applications.
  Optimal dilutions/concentrations should be determined by the end user.

Target

• RelevanceAgrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors （nAChRs） ａnd other proteins during development at the neuromuscular junction. Agrin, MuSK ａnd Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface ａnd induces AChR aggregation. The central region contains two O-linked glycosylation sites ａnd a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain ａnd glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.
• Cellular localizationSecreted; extracellular space; extracellular matrix. Note: Synaptic basal lamina at the neuromuscular junction.
• Database links
  • Entrez Gene: 375790 Human
  • Entrez Gene: 11603 Mouse
  • Entrez Gene: 25592 Rat
  • Omim: 103320 Human
  • SwissProt: O00468 Human
  • SwissProt: A2ASQ1 Mouse
  • SwissProt: P25304 Rat
  • Unigene: 273330 Human

  see all
• Alternative names
  • AGRIN antibody
  • Agrin proteoglycan antibody
  • AGRN antibody

  see all

Anti-Agrin antibody images

• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Agrin antibody （ab85174）
  ab85174, at 1/100 dilution, staining Agrin in formalin-fixed, paraffin-embedded human skeletal muscle by Immunohistochemistry.

References for Anti-Agrin antibody （ab85174）