Anti-Aggrecan，软骨蛋白聚糖/可聚蛋白多糖抗体

• Product nameAnti-Aggrecan antibody [6-B-4]See all Aggrecan primary antibodies ...
• Description
  Mouse monoclonal [6-B-4] to Aggrecan
• SpecificityRecognises the linear amino acid sequence 394EPEEPFTFAPEI406 present in the interglobular domain （IGD） of human ａnd bovine aggrecan （in both intact ａnd matrix protease degraded aggrecan） but not pig ａnd several other animals.
• Tested applicationsELISA, IHC-P, IHC-Fr, WB, ICC/IF more details
• Species reactivity
  Reacts with: Cow, Human
• Immunogen

  Synthetic peptide: EPEEPFTFAPEI, corresponding to amino acids 413-424 of Human Aggrecan.

  Run BLAST with Run BLAST with
• General notesSamples are usually deglycosylated using 0.01 Units Chondroitinase ABC （Sigma）, 0.01 Units Keratanase （Seikagaku） ａnd 0.0001 Units Keratanase II （Seikagaku） per 10µg S-GAG of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE ａnd immunohistochemistry – see Little et al. ａnd Caterson et al. Diluted Technomouse culture supernatant.

  This antibody detects aggrecan metabolites （intact or matrix protease-catabolised） in human synovial fluid samples.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term （1-2 weeks）. Aliquot ａnd store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage buffer0.2M Tris/HCl pH 7.4, 1-10% foetal calf serum, 0.02% sodium azide
• PurityTissue culture supernatant
• Primary antibody notesThis antibody detects aggrecan metabolites （intact or matrix protease-catabolised） in human synovial fluid samples.
• ClonalityMonoclonal
• Clone number6-B-4
• Myelomax63-Ag8.653
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Aggrecan

  • Stem Cells
  • Mesenchymal Stem Cells
  • Chondrogenesis

Applications

Target

• RelevanceAggrecan is a member of a family of large, aggregating proteoglycans （also including versican, brevican ａnd neurocan） which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, ａnd G3. Between the G1 ａnd G2 domains there is an interglobulin region （IGD）. The IGD region is the major site of cleavage by specific proteases like metalloproteinases （MMPs） ａnd aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis ａnd osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, ａnd axonal outgrowth in the CNS.
• Cellular localizationSecreted; extracellular matrix.
• Database links
  • Entrez Gene: 280985 Cow
  • Entrez Gene: 176 Human
  • Omim: 155760 Human
  • SwissProt: P13608 Cow
  • SwissProt: P16112 Human
  • Unigene: 2159 Human
• Alternative names
  • ACAN antibody
  • AGC 1 antibody
  • AGC1 antibody

  see all

Anti-Aggrecan antibody [6-B-4] images

• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - Aggrecan antibody [6-B-4] （ab3778）This image is courtesy of an Abreview submitted by Ms Elizabeth Chlipala
  ab3778 at 1/100 staining human articular cartilage by IHC-P. The tissue was formaldehyde fixed ａnd blocked with a serum free protein block. An enzymatic antigen retrieval step was performed ａnd the tissue was then incubated with the antibody for 1 hour. An HRP conjugated goat polyclonal antibody was used as the secondary.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Aggrecan antibody [6-B-4] （ab3778）Image from Limin Wang, M.S et al, TISSUE ENGINEERING: Part A Volume 15, Number 5, 2009, Fig 5.
  DOI: 10.1089=ten.tea.2008.0012
  ab3778 used at a 1/50 dilution staining Aggrecan in human umbilical cord matrix mesenchymal stromal cells by Immunocytochemistry/ Immunofluorescence.
  
  Frozen sections of the 3D constructs （10µm） （n=2） were rehydrated with PBS for 5 minutes, ａnd endogenous peroxidase activity was inhibited using 1% hydrogen peroxide in methanol for 30 minutes. The sections were then blocked in 3% horse serum for 20 minutes ａnd incubated with a primary antibody, ab3778, for 1 hour at a 1/50 dilution. After primary antibody incubation the sections were incubated with a streptavidin linked horse anti-mouse IgG secondary antibody for 30 minutes. After secondary antibody incubation, the sections were incubated with avidin-biotinylated enzyme complex for 30 minutes, ａnd then VIP substrate （purple color） was applied on sections for 4 minutes.
  
  The image shown is at 4 weeks, 25M.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Aggrecan antibody [6-B-4] （ab3778）Image from Berg V et al., Arthritis Res Ther. 2010;12（6）:R228. Epub 2010 Dec 30. Fig 1.; doi:10.1186/ar3215; 30 December 2010, Arthritis Research & Therapy 2010, 12:R228
  Immunofluorescence analysis of Human articular chondrocytes, staining Aggrecan with ab3778.
  
  Cells were fixed with paraformaldehyde ａnd blocked with 1% BSA for 1 hour. Cells were incubated with primary antibody overnight at 4°C. An AlexaFluor®488-conjugated rabbit anti-mouse IgG was used as the secondary antibody.

References for Anti-Aggrecan antibody [6-B-4] （ab3778）