Anti-AGEs，晚期糖基化终末产物抗体

• Product nameAnti-AGE antibodySee all AGE primary antibodies ...
• Description
  Rabbit polyclonal to AGE
• Specificityab23722 reacts with Advanced Glycation End Products （AGE）, Cross-reacts with BSA ａnd HSA < 1%
• Tested applicationsWB, ICC/IF, ELISA, IHC-Fr, IHC-P more details
• Immunogen

  Advanced Glycation End Products （BSA-AGE ａnd HSA-AGE）

• Positive controlHuman lens, arteriosclerotic plaques
• General notes

  ab23722 is suitable for the detection of different AGE products in tissues, tissue extracts ａnd body fluids.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot ａnd store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 0.85% Sodium chloride, 0.05M PBS, pH 7.15
• Concentration200 µg at 1 mg/ml
  
• PurityProtein A purified
• Primary antibody notesab23722 is suitable for the detection of different AGE products in tissues, tissue extracts ａnd body fluids.
• ClonalityPolyclonal
• IsotypeIgG
• Research Areas
  • Immunology
  • Immune System Diseases
  • Autoimmune

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Cardiovascular
  • Atherosclerosis
  • Lipoprotein metabolism

  • Cardiovascular
  • Atherosclerosis
  • Diabetes associated

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids ａnd lipoproteins

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Integration of energy metabolism

  • Metabolism
  • Pathways ａnd Processes
  • Metabolic signaling pathways
  • Lipid ａnd lipoprotein metabolism
  • Lipid metabolism

  • Metabolism
  • Pathways ａnd Processes
  • Metabolic signaling pathways
  • Lipid ａnd lipoprotein metabolism
  • Lipoprotein metabolism

  • Metabolism
  • Pathways ａnd Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Metabolism
  • Pathways ａnd Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Integration of energy

  • Metabolism
  • Types of disease
  • Diabetes

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Heart disease

Applications

Target

• RelevanceThe non enzymatic reaction of reducing carbohydrates with lysine side chains ａnd N terminal amino groups of macromolecules （amino acids, proteins, phospholipids ａnd nucleic acids） is called the Maillard reaction or glycation. The latter products of this process, termed advanced glycation end products （AGEs）, adversely affect the functional properties of proteins, lipids ａnd DNA. In long lived tissue proteins, these chemical modifications accumulate with age ａnd may contribute to the pathophysiology of ageing ａnd long term complications of diabetes, atherosclerosis ａnd renal failure.
• Cellular localizationCell Membrane ａnd Secreted
• Alternative names
  • Advanced glycation end products antibody
  • AGEs antibody

Anti-AGE antibody images

• 

  Western blot - Anti-AGE antibody （ab23722）This image is courtesy of an Abreview submitted by Josephine Böhme
  All lanes : Anti-AGE antibody （ab23722） at 1/1000 dilution
  
  Lane 1 : Mouse Brain Lysate（Brain ａnd Hippocampus） at 25 µg
  Lane 2 : Glycated BSA at 15 µg
  
  Secondary
  HRP-conjugated Goat anti-rabbit IgG at 1/800 dilution
  
  Performed under reducing conditions.

  This image is courtesy of an Abreview submitted by Josephine Böhme

  12% SDS-PAGE gel run using denaturing conditions. Membrane blocked with 5% BSA for 30 minutes at room temperature. Primary antibody diluted in 5% BSA in TBS-T ａnd incubated for 20 hours in a fridge

  See Abreview

• 

  Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections）-AGE antibody（ab23722）
  ab23722 （1µg/ml） staining AGE in human colon using an automated system （DAKO Autostainer Plus）. Using this protocol there is strong staining of both cell membrane ａnd cytoplasm.
  Sections were rehydrated ａnd antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes （containing casein 0.25% in PBS） then incubated with primary antibody for 20 min ａnd detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin ａnd coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration ａnd incubation time is recommended. Signal amplification may be required.
  
• 

  Immunocytochemistry/ Immunofluorescence-AGE antibody（ab23722）
  ICC/IF image of ab23722 stained HepG2 cells. The cells were 4% formaldehyde fixed （10 min） ａnd then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells ａnd block non-specific protein-protein interactions. The cells were then incubated with the antibody （ab23722, 5µg/ml） overnight at +4°C. The secondary antibody （green） was Alexa Fluor® 488 goat anti-rabbit IgG （H+L） used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes （red） at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei （blue） at a concentration of 1.43µM.
  
  

References for Anti-AGE antibody （ab23722）